Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 640212
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Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Complement Factor H-related 4/CFHR4
Glu20-Glu331
Accession # Q92496

Specificity

Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CFHR1, rhCFHR2, or rhCFHR5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Detection of Complement Factor H-related 4/CFHR4 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of Complement Factor H‑related 4/CFHR4 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Complement Factor H-related 4/CFHR4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC5980G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Intracellular Molecules.

Applications

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Complement Factor H-related 4/CFHR4

FHR4 (Complement factor H-related protein 4), also known as CFHR4, is an 84-86 kDa glycoprotein member of group 2 in the  factor H-related protein family of molecules. It is synthesized by hepatocytes and circulates in plasma as a monomer. FHR4 binds to lipoproteins (LDL and VLDL), C3b and C3d, and to native (pentameric) C-reactive protein (pCRP). In addition to full-length 86 kDa CFHR4 (called FHR4A), there are two alternative splice forms. One simply shows a deletion of Glu20 relative to the full-length form. A second called FHR4B is 42-45 kDa in size, and shows a deletion of amino acids (aa) 60-306 within the mature CFHR4 molecule. This short isoform is the molecule used as the immunogen for clone 640212. This 312 aa short form contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. Both major isoforms appear to show the same activity. That is, they serve as a platform for C3 convertase assembly, and bind pCRP which leads to classical complement activation. Over aa 20-331, human CFHRB shares 47% aa identity with a related molecule found in rat.

Alternate Names

CFHL4, CFHR4, Complement Factor Hrelated 4, FHR4

Entrez Gene IDs

10877 (Human)

Gene Symbol

CFHR4

UniProt

Additional Complement Factor H-related 4/CFHR4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Citations for Human Complement Factor H‑related 4/CFHR4 Alexa Fluor® 488‑conjugated Antibody

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