Human Complement Factor H-related 4/CFHR4 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC5980G-100UG
Human Complement Factor H-related 4/CFHR4 Alexa Fluor® 488-conjugated Antibody in Data
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Human Complement Factor H-related 4/CFHR4 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CFHR1, rhCFHR2, or rhCFHR5 is observed.
Source
Monoclonal Mouse IgG1 Clone # 640212
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Complement Factor H-related 4/CFHR4
Glu20-Glu331
Accession # Q92496
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Complement Factor H‑related 4/CFHR4 in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of Complement Factor H‑related 4/CFHR4 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Complement Factor H-related 4/CFHR4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC5980G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Complement Factor H-related 4/CFHR4

FHR4 (Complement factor H-related protein 4), also known as CFHR4, is an 84-86 kDa glycoprotein member of group 2 in the  factor H-related protein family of molecules. It is synthesized by hepatocytes and circulates in plasma as a monomer. FHR4 binds to lipoproteins (LDL and VLDL), C3b and C3d, and to native (pentameric) C-reactive protein (pCRP). In addition to full-length 86 kDa CFHR4 (called FHR4A), there are two alternative splice forms. One simply shows a deletion of Glu20 relative to the full-length form. A second called FHR4B is 42-45 kDa in size, and shows a deletion of amino acids (aa) 60-306 within the mature CFHR4 molecule. This short isoform is the molecule used as the immunogen for clone 640212. This 312 aa short form contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. Both major isoforms appear to show the same activity. That is, they serve as a platform for C3 convertase assembly, and bind pCRP which leads to classical complement activation. Over aa 20-331, human CFHRB shares 47% aa identity with a related molecule found in rat.

Entrez Gene IDs
10877 (Human)
Alternate Names
complement factor H-related protein 4; CFHL4; CFHR4; Complement Factor Hrelated 4; Complement Factor H-related 4; FHR4; FHR-4

Product Datasheets

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