Human Complement Factor H-related 4/
CFHR4 Antibody Summary
Accession # Q92496
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Complement Factor H-related 4/CFHR4 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Complement Factor H-related 4/ CFHR4 at approximately 45 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Complement Factor H-related 4/CFHR4 in Human PBMCs. Complement Factor H-related 4/CFHR4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMC) using Human Complement Factor H-related 4 Monoclonal Antibody (Catalog # MAB5980) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Complement Factor H-related 4/CFHR4 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Human Complement Factor H-related 4/CFHR4 Monoclonal Antibody (Catalog # MAB5980, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor H-related 4/CFHR4
FHR4 (Complement factor H-related protein 4; also FHR4B and CFHR4) is a 45 kDa glycoprotein member of the factor H-related protein family. It is synthesized by hepatocytes and circulates in plasma. FHR4(B) binds to C3b and C3d. It also binds to native (pentameric) C-reactive protein (pCRP), and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance. Mature human CFHR is 312 amino acids (aa) in length. It contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. There is a circulating, 86 kDa alternative splice form termed FHR4A that shows an insertion of 247 aa after Asp92. This is considered the dominant isoform, and appears to show the same activity as CFHR. Over aa 41-331, human CFHR shares 61% aa identity with a related molecule found in rat.
Citation for Human Complement Factor H-related 4/
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Factor H-related protein 4 activates complement by serving as a platform for the assembly of alternative pathway C3 convertase via its interaction with C3b protein.
Authors: Hebecker M, Jozsi M
J. Biol. Chem., 2012;287(23):19528-36.
Sample Types: Serum
Applications: ELISA Development
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