Human Connexin 43/GJA1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7737A
Detection of Connexin 43/GJA1 in HEK293 Human Cell Line Transfected with Human Connexin 43/GJA1 and eGFP by Flow Cytometry.
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Citations (7)
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Human Connexin 43/GJA1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Connexin 43/GJA1 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 578618
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0 transfected with human Connexin 43/GJA1
Accession # P17302
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Connexin 43/GJA1 antibody in HEK293 Human Cell Line Transfected with Human Connexin 43/GJA1 and eGFP antibody by Flow Cytometry. View Larger

Detection of Connexin 43/GJA1 in HEK293 Human Cell Line Transfected with Human Connexin 43/GJA1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Connexin 43/GJA1 and eGFP was stained with and either (A) Mouse Anti-Human Connexin 43/GJA1 APC-conjugated Monoclonal Antibody (Catalog # FAB7737A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Connexin 43/GJA1

Connexin43 (Cx43; also gap junction alpha -1 protein) is a 41 - 44 kDa member of the connexin family, alpha -type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.

References
  1. Sohl, G. and K. Willecke (2004) Cardiovasc. Res. 62:228.
  2. Willecke, K. et al. (2002) Biol. Chem. 383:725.
  3. Giepmans, B.N.G. (2004) Cardiovasc. Res. 62:233.
  4. Vinken, M. et al. (2006) Cell. Signal. 18:592.
  5. Stout, C. et al. (2004) Curr. Opin. Cell Biol. 16:507.
  6. Bao, X. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C647.
  7. Yogo, K. et al. (2006) J. Reprod. Dev. 52:321.
Entrez Gene IDs
2697 (Human)
Alternate Names
Connexin 43; connexin-43; CX43; CX43gap junction protein, alpha-like; DFNB38; Gap junction 43 kDa heart protein; gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa (connexin 43); gap junction protein, alpha 1, 43kDa; GJA1; GJAL; HSS; ODD; ODDD; ODOD; SDTY3

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Citations for Human Connexin 43/GJA1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Solute exchange through gap junctions lessens the adverse effects of inactivating mutations in metabolite-handling genes
    Authors: Monterisi S, Michl J, Hulikova A et al.
    eLife
  2. Senolytic activity of small molecular polyphenols from olive restores chondrocyte redifferentiation and promotes a pro-regenerative environment in osteoarthritis
    Authors: Marta Varela-Eirín, Paula Carpintero-Fernández, Agustín Sánchez-Temprano, Adrián Varela-Vázquez, Carlos Luis Paíno, Antonio Casado-Díaz et al.
    Aging (Albany NY)
  3. Interaction between insulin and androgen signalling in decidualization, cell migration and trophoblast invasion in vitro
    Authors: AL Hirschberg, I Jakson, C Graells Br, D Salamon, D Ujvari
    Journal of Cellular and Molecular Medicine, 2021-08-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Soluble silica stimulates osteogenic differentiation and gap junction communication in human dental follicle cells
    Authors: P Uribe, A Johansson, R Jugdaohsin, JJ Powell, C Magnusson, M Davila, A Westerlund, M Ransjö
    Sci Rep, 2020-06-18;10(1):9923.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The Analysis of In Vivo Aging in Human Bone Marrow Mesenchymal Stromal Cells Using Colony-Forming Unit-Fibroblast Assay and the CD45lowCD271+ Phenotype
    Authors: P Ganguly, JJ El-Jawhari, AN Burska, F Ponchel, PV Giannoudis, EA Jones
    Stem Cells Int, 2019-08-01;2019(0):5197983.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Targeting of chondrocyte plasticity via connexin43 modulation attenuates cellular senescence and fosters a pro-regenerative environment in osteoarthritis
    Authors: M Varela-Eir, A Varela-Váz, A Guitián-Ca, CL Paíno, V Mato, R Largo, T Aasen, A Tabernero, E Fonseca, M Kandouz, JR Caeiro, A Blanco, MD Mayán
    Cell Death Dis, 2018-12-05;9(12):1166.
    Species: Human
    Sample Types: Whole Cells
  7. In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells
    Authors: P Szaraz, M Librach, L Maghen, F Iqbal, TA Barretto, S Kenigsberg, A Gauthier-F, CL Librach
    Stem Cells Int, 2016-03-30;2016(0):7513252.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human Connexin 43/GJA1 APC-conjugated Antibody
By Marina Rodriguez on 05/12/2021
Application: Flow Sample Tested: HCC1143,HCC1937 cell line Species: Human

Human breast cancer HCC1143 cells were engineered to overexpress Cx43, as they naturally lack it. They were also permeabilized to detect both external and internal Cx43. The red graph shows the unstained control cells, and the blue graph the Cx43-APC-stained cells. Events=30k