Human Connexin 43/GJA1 APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Connexin 43/GJA1 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 578618
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0 transfected with human Connexin 43/GJA1
    Accession # P17302
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Connexin 43/GJA1 in HEK293 Human Cell Line Transfected with Human Connexin 43/GJA1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Connexin 43/GJA1 and eGFP was stained with and either (A) Mouse Anti-Human Connexin 43/GJA1 APC‑conjugated Monoclonal Antibody (Catalog # FAB7737A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Connexin 43/GJA1
Connexin43 (Cx43; also gap junction alpha -1 protein) is a 41 - 44 kDa member of the connexin family, alpha -type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.
  • References:
    1. Sohl, G. and K. Willecke (2004) Cardiovasc. Res. 62:228.
    2. Willecke, K. et al. (2002) Biol. Chem. 383:725.
    3. Giepmans, B.N.G. (2004) Cardiovasc. Res. 62:233.
    4. Vinken, M. et al. (2006) Cell. Signal. 18:592.
    5. Stout, C. et al. (2004) Curr. Opin. Cell Biol. 16:507.
    6. Bao, X. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C647.
    7. Yogo, K. et al. (2006) J. Reprod. Dev. 52:321.
  • Entrez Gene IDs:
    2697 (Human)
  • Alternate Names:
    Connexin 43; connexin-43; CX43; CX43gap junction protein, alpha-like; DFNB38; Gap junction 43 kDa heart protein; gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa (connexin 43); gap junction protein, alpha 1, 43kDa; GJA1; GJAL; HSS; ODD; ODDD; ODOD; SDTY3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells
    Authors: P Szaraz, M Librach, L Maghen, F Iqbal, TA Barretto, S Kenigsberg, A Gauthier-F, CL Librach
    Stem Cells Int, 2016;2016(0):7513252.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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