Detection of Connexin 43/GJA1 in HEK293 Human Cell Line Transfected with Human Connexin 43/GJA1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human Connexin 43/GJA1 and eGFP was stained with and either (A) Mouse Anti-Human Connexin 43/GJA1 APC‑conjugated Monoclonal Antibody (Catalog # FAB7737A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Connexin 43/GJA1
Connexin43 (Cx43; also gap junction alpha -1 protein) is a 41 - 44 kDa member of the connexin family, alpha -type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.
Sohl, G. and K. Willecke (2004) Cardiovasc. Res. 62:228.
Willecke, K. et al. (2002) Biol. Chem. 383:725.
Giepmans, B.N.G. (2004) Cardiovasc. Res. 62:233.
Vinken, M. et al. (2006) Cell. Signal. 18:592.
Stout, C. et al. (2004) Curr. Opin. Cell Biol. 16:507.
Bao, X. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C647.
Yogo, K. et al. (2006) J. Reprod. Dev. 52:321.
Entrez Gene IDs:
Connexin 43; connexin-43; CX43; CX43gap junction protein, alpha-like; DFNB38; Gap junction 43 kDa heart protein; gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa (connexin 43); gap junction protein, alpha 1, 43kDa; GJA1; GJAL; HSS; ODD; ODDD; ODOD; SDTY3
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