Human CRISP-2 Biotinylated Antibody

Catalog #: BAF2575 Datasheet / COA / SDS

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BAF2575

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Human CRISP-2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CRISP-2 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human CRISP-3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CRISP-2
Lys22-Tyr243
Accession # P16562

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CRISP‑2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-2

Human CRISP-2, the ortholog of rodent testis specific protein 1 (Tpx1) is a secreted protein expressed in testis. It is a cell adhesion molecule that mediates interactions between spermatogenic cells and Sertoli cells. Mature human CRISP-2 shares 86%, 73% and 71% amino acid sequence identity with the equine, mouse and rat proteins, respectively. Human CRISP-2 also shares 71% amino acid sequence homology with human CRISP-3.

Long Name

Cysteine-rich Secretory Protein 2

Entrez Gene IDs

7180 (Human); 22024 (Mouse)

Alternate Names

Cancer/testis antigen 36; CRISP2; CRISP-2; CRISP-2GAPDL5; CT36TSP1; cysteine-rich secretory protein 2; GAPDL5; glyceraldehyde-3-phosphate dehydrogenase-like 5; testis specific protein 1 (probe H4-1 p3-1); Testis-specific protein TPX-1; TPX1; TPX1MGC111136; TSP1