Human CXCL10/IP-10 Quantikine QuicKit ELISA

For use with catalog number QK206
Catalog # Availability Size / Price Qty
QK266
Control Products Available
Human IP-10 ELISA Standard Curve
2 Images
Product Details
Procedure
FAQs
Supplemental Products
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Human CXCL10/IP-10 Quantikine QuicKit ELISA Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), Plasma (25 uL)
Sensitivity
4.1 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Plasma)
Specificity
Natural and recombinant human IP-10.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® QuicKit™ Human CXCL10/IP-10 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IP-10 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IP-10 and antibodies raised against the recombinant protein. Results obtained using natural human IP-10 showed linear curves that were parallel to the standard curves obtained using the QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human IP-10.

Precision

Intra-Assay Precision (Precision within an assay) <div>Two samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) <div>Two samples of known concentration were tested in ten separate assays to assess&nbsp;inter-assay precision.</div>

Cell Culture Supernates, Serum, Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 10 10
Mean 96.7 521 95.3 515
Standard Deviation 1.12 10.6 10.8 42.1
CV% 1.2 2 11.3 8.2

Recovery

The recovery of human IP-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 119 110-129
EDTA Plasma (n=2) 86 78-95
Heparin Plasma (n=2) 104 92-118
Serum (n=2) 71 67-76

Data Examples

Human IP-10 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL10/IP-10/CRG-2

IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.

Entrez Gene IDs:
3627 (Human); 15945 (Mouse)
Alternate Names:
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

Assay Procedure

These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.

QuicKit ELISA assay procedure

FAQs

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ELISA Controls

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