Human CXCL10/IP-10 Quantikine QuicKit ELISA Summary
Cell Culture Supernates, Serum, Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human IP-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||119||110-129|
|EDTA Plasma (n=2)||86||78-95|
|Heparin Plasma (n=2)||104||92-118|
Human IP-10 ELISA Standard Curve
Preparation and Storage
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.
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