Human CXCL10/IP-10 Quantikine QuicKit ELISA
Human CXCL10/IP-10 Quantikine QuicKit ELISA Summary
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Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 96.7 | 521 | 95.3 | 515 |
| Standard Deviation | 1.12 | 10.6 | 10.8 | 42.1 |
| CV% | 1.2 | 2 | 11.3 | 8.2 |
Recovery
The recovery of human IP-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 119 | 110-129 |
| EDTA Plasma (n=2) | 86 | 78-95 |
| Heparin Plasma (n=2) | 104 | 92-118 |
| Serum (n=2) | 71 | 67-76 |
Scientific Data
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Human IP-10 QuicKit Spiked Recovery Competitor Comparison IP-10 is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Plasma recovery is 95% compared to 49% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
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Human IP-10 QuicKit Spiked Linearity Competitor Comparison IP-10 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-124% compared to 112%-183% for the top competitor.
Product Datasheets
Preparation and Storage
Background: CXCL10/IP-10/CRG-2
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
Assay Procedure
These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.
FAQs
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