Human CXCL10/IP-10 Quantikine QuicKit ELISA Summary
Complete Your Research
Complete Your Experiment
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of human IP-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||119||110-129|
|EDTA Plasma (n=2)||86||78-95|
|Heparin Plasma (n=2)||104||92-118|
Human IP-10 ELISA Standard Curve
Human IP-10 QuicKit Spiked Recovery Competitor Comparison IP-10 is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Plasma recovery is 95% compared to 49% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
Human IP-10 QuicKit Spiked Linearity Competitor Comparison IP-10 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-124% compared to 112%-183% for the top competitor.
Preparation and Storage
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.
No product specific FAQs exist for this product, however you mayView all ELISA FAQs
Reviews for Human CXCL10/IP-10 Quantikine QuicKit ELISA
There are currently no reviews for this product. Be the first to review Human CXCL10/IP-10 Quantikine QuicKit ELISA and earn rewards!
Have you used Human CXCL10/IP-10 Quantikine QuicKit ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image