Human CXCL5/ENA-78 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC654R-100UG
Detection of CXCL5/ENA-78 in Human PBMC by FlowCytometry
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Human CXCL5/ENA-78 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL5/ENA-78 in ELISAs. Does not cross-react with recombinant human CXCL1, 2, or 3.
Source
Monoclonal Mouse IgG1 Clone # 33170
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CXCL5/ENA-78
Ala37-Asn114
Accession # P42830
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of CXCL5/ENA-78 in Human PBMC by FlowCytometry View Larger

Detection of CXCL5/ENA-78 in Human PBMC by FlowCytometry Human PBMC treated with LPS were stainedwith Mouse Anti-HumanCXCL5/ENA-78 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog# IC654R, filled histogram) or isotype control antibody (Catalog # IC002R,open histogram). To facilitate intracellular staining, cells were fixed withFlow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL5/ENA-78

CXCL5, also known as ENA-78, is a CXC chemokine that plays a role in inflammatory responses and angiogenesis.

Entrez Gene IDs
6374 (Human)
Alternate Names
CXCL5; CXCL5/ENA-78; ENA78; ENA-78

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