Human CXCL5/ENA-78 Alexa Fluor® 647-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL5/ENA-78 in ELISAs. Does not cross-react with recombinant human CXCL1, 2, or 3.
  • Source
    Monoclonal Mouse IgG1 Clone # 33170
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CXCL5/ENA-78
    Ala37-Asn114
    Accession # P42830
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of CXCL5/ENA-78 in Human PBMC by FlowCytometry Human PBMC treated with LPS were stainedwith Mouse Anti-HumanCXCL5/ENA-78 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog# IC654R, filled histogram) or isotype control antibody (Catalog # IC002R,open histogram). To facilitate intracellular staining, cells were fixed withFlow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCL5/ENA-78
CXCL5, also known as ENA-78, is a CXC chemokine that plays a role in inflammatory responses and angiogenesis.
  • Entrez Gene IDs:
    6374 (Human)
  • Alternate Names:
    CXCL5; CXCL5/ENA-78; ENA78; ENA-78
Related Research Areas

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