Detection of CXCL5/ENA-78 in Human PBMC by FlowCytometry
Human PBMC treated with LPS were stainedwith Mouse Anti-HumanCXCL5/ENA-78 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog# IC654R, filled histogram) or isotype control antibody (Catalog # IC002R,open histogram). To facilitate intracellular staining, cells were fixed withFlow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CXCL5, also known as ENA-78, is a CXC chemokine that plays a role in inflammatory responses and angiogenesis.
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