Human CXCR2/IL-8RB Fluorescein-conjugated Antibody

Clone 48311 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB331F-025
FAB331F-100
Human CXCR2/IL-8RB Fluorescein-conjugated Antibody in Data
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Product Details
Citations (7)
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Human CXCR2/IL-8RB Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).
Source
Monoclonal Mouse IgG2A Clone # 48311
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB
Met1-Leu355
Accession # AAB25880
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CXCR2/IL-8 RB in Human Peripheral Blood Granulocytes by Flow Cytometry. View Larger

Detection of CXCR2/IL-8 RB in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR2/IL-8 RB Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB331F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR2/IL-8RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Long Name
Interleukin 8 Receptor B
Entrez Gene IDs
3579 (Human); 12765 (Mouse); 29385 (Rat)
Alternate Names
CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; CMKAR2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 RB; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B

Product Datasheets

Citations for Human CXCR2/IL-8RB Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  2. Impaired neutrophil extracellular trap formation: a novel defect in the innate immune system of aged individuals.
    Authors: Hazeldine J, Harris P, Chapple I, Grant M, Greenwood H, Livesey A, Sapey E, Lord J
    Aging Cell, 2014;13(4):690-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
    Authors: Eikawa S, Ohue Y, Kitaoka K
    J. Immunol., 2010;185(11):6734-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis.
    Authors: Yang G, Rosen DG, Liu G
    Clin. Cancer Res., 2010;16(15):3875-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. HIF-1 and NF-kappaB-mediated upregulation of CXCR1 and CXCR2 expression promotes cell survival in hypoxic prostate cancer cells.
    Authors: Maxwell PJ, Gallagher R, Seaton A, Wilson C, Scullin P, Pettigrew J, Stratford IJ, Williams KJ, Johnston PG, Waugh DJ
    Oncogene, 2007;26(52):7333-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Human CXCR2 (hCXCR2) takes over functionalities of its murine homolog in hCXCR2 knockin mice.
    Authors: Mihara K, Smit MJ, Krajnc-Franken M, Gossen J, Rooseboom M, Dokter W
    Eur. J. Immunol., 2005;35(9):2573-82.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.
    Authors: Liu L, Xie F, Chang K, Li M, Meng Y, Wang X, Li H, Li D, Yu J
    Int J Clin Exp Pathol, 0;7(2):575-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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