Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Guinea Pig, Rabbit

Applications

Validated:

Immunohistochemistry, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, ELISA Development, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 48311
View all CXCR2/IL-8RB Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB
Met1-Leu355
Accession # AAB25880

Specificity

Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCR2/IL-8RB Antibody

Detection of CXCR2/IL-8 RB antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of CXCR2/IL‑8 RB in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
CXCR2/IL‑8 RB antibody in Human Lymph Node by Immunohistochemistry (IHC-P).

CXCR2/IL‑8 RB in Human Lymph Node.

CXCR2/IL-8 RB was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCR2/IL‑8 RB Antibody.

Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCR2/IL‑8 RB Antibody.

Recombinant Human CXCL1/GROa (Catalog # 275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/GROa (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331). The ND50 is typically 1-5 µg/mL.
Detection of CXCR2/IL-8RB by Western Blot

Detection of CXCR2/IL-8RB by Western Blot

SW620 cell-secreted factors require CXCR2 signaling pathway to induce the endothelial Cx32 expression and tube formationA. IL-8 secretion in conditioned media from SW480 and SW620 cells was examined through ELISA. CRC cells were exposed or not to the HMEC-CM. All cell media were collected after 6 h (mean ± SD, *P-values < 0.01 Mann-Whitney U test and Kruskal-Wallis test; n = 4). B. SW620-CM increase the endothelial expression of the CXCR2 receptor. A slight but significant increase in optical density (OD; relative to control) of bands was detected in response to SW620-CM compared with unstimulated HMEC (P-values < 0.01 Mann-Whitney U test and Kruskal-Wallis test; n = 4). No inhibitory effect was observed by pre-treating HMEC with SB225002 (200 nM), the CXCR2 antagonist. Representative of 4 experiments (Hsc70 as loading control; 100 μg/lane). C–D. Endothelial CXCR2 conveys angiogenic effects of SW620-CM. HMEC were pretreated or not with neutralizing anti-CXCR2 antibody (anti-CXCR2 mAb; 10 μg/ml) or SB225002. Cells were exposed to SW620-CM or human recombinant rhIL-8 (1 ng/ml) for 6 h. C. Representative Images of tube formation (Bar 80 μm). The dotted areas are enlarged in the inserts on the right. Arrows indicated branch points. D. Number of branch points per field of view was quantified (mean ± SD, n = 4; *P < 0.01 vs control). E. Blocking CXCR2 significantly diminished SW620-CM-induced expression of Cx32 in HMEC (P-values < 0.01 vs SW620-CM Mann-Whitney U test and Kruskal-Wallis test; n = 3). HMEC were exposed to cell-conditioned media for 6 h. In some cases, HMEC were pretreated with anti-CXCR2 mAb or SB225002, as indicated. This is a representative of three experiments with similar results (Hsc70 as loading control; 100 μg/lane). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26320187), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CXCR2/IL-8RB by Flow Cytometry

Detection of CXCR2/IL-8RB by Flow Cytometry

AESIS-1 enhances migration activities and CXCR2 expression through ERK and p38 MAPK signaling in HDF cells. Cells were treated with 20 ng/mL AESIS-1 for 24 h. MAPK inhibitors (ERK-PD98059, 10 μM; p38-SB203580, 10 μM; and JNK-SP600215, 20 μM) were added to cells 1 h before peptide treatment. (a) Transwell migration assay was performed using MAPK inhibitors and AESIS-1-treated HDF cells for 12 incubation hours with or without CXCL8 in lower chamber. Error bars, mean ± SD. One-way ANOVA with Turkey’s multiple comparisons test was used for statistical analysis. * p < 0.05, *** p < 0.001 (versus AESIS-1-treated group, with CXCL8-treated control); §§§ p < 0.001 (versus without AESIS-1-treated group, with CXCL8-treated group); ### p < 0.001 (versus without AESIS-1-, CXCL8-treated groups). (b) Surface protein expression of CXCR2 was measured with flow cytometric analysis after HDF cells were treated with AESIS-1 for 24 h with or without MAPK inhibitor pre-treatment. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/7/3937), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR2/IL-8RB Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood granulocytes

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 ng/mL Recombinant Human CXCL1/GRO alpha /KC/CINC‑1.

Reviewed Applications

Read 6 reviews rated 4.3 using MAB331 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR2/IL-8RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Long Name

Interleukin 8 Receptor B

Alternate Names

CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB

Entrez Gene IDs

3579 (Human); 12765 (Mouse); 29385 (Rat)

Gene Symbol

CXCR2

UniProt

AAB25880

Additional CXCR2/IL-8RB Products

Product Documents for Human CXCR2/IL-8RB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CXCR2/IL-8RB Antibody

For research use only

Citations for Human CXCR2/IL-8RB Antibody

Customer Reviews for Human CXCR2/IL-8RB Antibody (6)

4.3 out of 5
6 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 5 of 6 reviews Showing All
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  • Human CXCR2/IL-8RB Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Myofibroblasts
    Species: Human
    Verified Customer | Posted 08/05/2021
    Human CXCR2/IL-8RB Antibody MAB331
  • Human CXCR2/IL-8 RB Antibody
    Name: Michelle Chen
    Application: Block/Neutralize
    Sample Tested: Peripheral blood neutrophils
    Species: Human
    Verified Customer | Posted 04/26/2017
  • Human CXCR2/IL-8 RB Antibody
    Name: Ruhul Choudhury
    Application: Immunohistochemistry
    Sample Tested: fetal membrane
    Species: Mouse
    Verified Customer | Posted 01/23/2017
    sodium citrate antigen retrieval
    Human CXCR2/IL-8RB Antibody MAB331
  • Human CXCR2/IL-8 RB Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 11/04/2016
    Human CXCR2/IL-8RB Antibody MAB331
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 22763101
    Species: Human
    Verified Customer | Posted 02/20/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 22626766
    Species: Human
    Verified Customer | Posted 02/20/2015

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