Human Cystatin C Quantikine ELISA Kit

(11 citations)
(1 Review)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL), Human Milk (50 uL)
  • Sensitivity
    0.227 ng/mL
  • Assay Range
    3.1 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
  • Specificity
    Natural and recombinant human Cystatin C
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC23, Quantikine Immunoassay Control Group 8 - Please Inquire
Product Summary
The Quantikine Human Cystatin C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cystatin C in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Cystatin C and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cystatin C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Cystatin C.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1.070.922.411.211.533.6


The recovery of Cystatin C spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 88-102
To assess the linearity of the assay, samples containing high concentrations of Cystatin C were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Cystatin C Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cystatin C
Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.
    • Entrez Gene IDs
      1471 (Human); 13010 (Mouse); 25307 (Rat);
    • Alternate Names
      ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    The conjugate must remain at 2-8 °C during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 3 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL cold Conjugate
    9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    11 Citations: Showing 1 - 10
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    Sample Type
    1. Identifying biomarkers of dementia prevalent among amnestic mild cognitively impaired ethnic female patients
      Alzheimers Res Ther, 2016;8(1):43.
      Species: Human
      Sample Type: Plasma
    2. A novel PKD1 variant demonstrates a disease-modifying role in trans with a truncating PKD1 mutation in patients with autosomal dominant polycystic kidney disease.
      Authors: Ali H, Hussain N, Naim M, Zayed M, Al-Mulla F, Kehinde E, Seaburg L, Sundsbak J, Harris P
      BMC Nephrol, 2015;16(0):26.
      Species: Human
      Sample Type: Serum
    3. Combined Biomarker Analysis for Risk of Acute Kidney Injury in Patients with ST-Segment Elevation Myocardial Infarction.
      Authors: Tung Y, Chang C, Chen Y, Chu P
      PLoS ONE, 2015;10(4):e0125282.
      Species: Human
      Sample Type: Serum
    4. Age-related changes of cystatin C expression and polarized secretion by retinal pigment epithelium: potential age-related macular degeneration links.
      Authors: Kay, Paul, Yang, Yit C, Hiscott, Paul, Gray, Donna, Maminishkis, Arvydas, Paraoan, Luminita
      Invest Ophthalmol Vis Sci, 2014;55(2):926-34.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Acute kidney injury biomarkers for patients in a coronary care unit: a prospective cohort study.
      Authors: Chen TH, Chang CH, Lin CY, Jenq CC, Chang MY, Tian YC, Hung CC, Fang JT, Yang CW, Wen MS, Lin FC, Chen YC
      PLoS ONE, 2012;7(2):e32328.
      Species: Human
      Sample Type: Serum
    6. Increased prevalence of albuminuria in HIV-infected adults with diabetes.
      Authors: Kim PS, Woods C, Dutcher L, Georgoff P, Rosenberg A, Mican JA, Kopp JB, Smith MA, Hadigan C
      PLoS ONE, 2011;6(9):e24610.
      Species: Human
      Sample Type: Serum
    7. Use of cystatin C-based estimations of glomerular filtration rate in patients with type 2 diabetes.
      Authors: Chudleigh RA, Ollerton RL, Dunseath G
      Diabetologia, 2009;52(7):1274-8.
      Species: Human
      Sample Type: Serum
    8. Cystatin C in cerebrospinal fluid as a biomarker of ALS.
      Authors: Tsuji-Akimoto S, Yabe I, Niino M, Kikuchi S, Sasaki H
      Neurosci. Lett., 2009;452(1):52-5.
      Species: Human
      Sample Type: CSF
    9. Imbalanced serum concentration between cathepsin B and cystatin C in patients with pelvic inflammatory disease.
      Authors: Tsai HT, Wang PH, Tee YT, Lin LY, Hsieh YS, Yang SF
      Fertil. Steril., 2009;91(2):549-55.
      Species: Human
      Sample Type: Serum
    10. A urinary biomarker profile for children with HIV-associated renal diseases.
      Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
      Kidney Int., 2009;76(2):207-14.
      Species: Human
      Sample Type: Urine
    11. Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
      Authors: Gunawardana CG, Kuk C, Smith CR
      J. Proteome Res., 2009;8(10):4705-13.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all 11 Citations
    ELISA Controls
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    Quantikine Immunoassay Control Group 8

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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Average Rating: 4 (Based on 1 review)

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    Sample TestedSerum,Urine

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