Human Cystatin C Quantikine ELISA Kit

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Human Cystatin C ELISA Standard Curve
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Product Details
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Citations (29)
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Human Cystatin C Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
0.227 ng/mL
Assay Range
3.1 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human Cystatin C
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human Cystatin C in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard Deviation (ng/mL)
Serum (n=36)792553-1257161
EDTA plasma (n=36)774560-1173155
Heparin plasma (n=36)786524-1284177
Saliva (n=11)1259103-31841077
Urine (n=12)62.912.6-18843.8

Cell Culture Supernates:
Human peripheral blood cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernate were removed and assayed for levels of human Cystatin C.

ConditionDay 1 (ng/mL)Day 5 (ng/mL)
UnstimulatedNDND
StimulatedND16
ND=Non-detectable

IMR-90 human lung fibroblast cells were cultured in MEM supplemented with 10% fetal bovine serum, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 0.1 mM sodium pyruvate. An aliquot of the cell culture supernate was assayed for human Cystatin C and measured 23.7 ng/mL.

MCF-7 human breast cancer cells were grown in DMEM/F12 media supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was assayed for human Cystatin C and measured 47.2 ng/mL.

HepG2 hepatocellular carcinoma cells were grown in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was assayed for human Cystatin C and measured 264 ng/mL.

Human Milk - Two human milk samples were assayed for human Cystatin C and measured 1456 ng/mL and 2431 ng/mL, respectively. 




Product Summary

The Quantikine Human Cystatin C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cystatin C in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Cystatin C and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cystatin C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Cystatin C.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 16.2 29.9 52.6 17.2 30.8 60.9
Standard Deviation 1.07 0.92 2.41 1.21 1.53 3.6
CV% 6.6 3.1 4.6 7 5 5.9

Recovery

The recovery of Cystatin C spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 88-102

Linearity

To assess the linearity of the assay, samples containing high concentrations of Cystatin C were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Cystatin C ELISA Linearity

Scientific Data

Human Cystatin C ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cystatin C

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.

Entrez Gene IDs:
1471 (Human); 13010 (Mouse); 25307 (Rat)
Alternate Names:
ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

The conjugate must remain at 2-8 °C during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 3 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL cold Conjugate
  9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Cystatin C Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. KIM-1, IL-18, and NGAL, in the Machine Learning Prediction of Kidney Injury among Children Undergoing Hematopoietic Stem Cell Transplantation-A Pilot Study
    Authors: Musia?, K;Stojanowski, J;Mi?kiewicz-Bujna, J;Ka?wak, K;Ussowicz, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  2. Optimizing Vancomycin Therapy in Critically Ill Children: A Population Pharmacokinetics Study to Inform Vancomycin Area under the Curve Estimation Using Novel Biomarkers
    Authors: Downes, KJ;Zuppa, AF;Sharova, A;Neely, MN;
    Pharmaceutics
    Species: Human
    Sample Types: Plasma, Urine
  3. Prenatal Metal Exposures and Associations with Kidney Injury Biomarkers in Children
    Authors: MD Politis, M Yao, C Gennings, M Tamayo-Ort, D Valvi, S Kim-Schulz, J Qi, C Amarasiriw, I Pantic, MC Tolentino, G Estrada-Gu, JH Greenberg, MM Téllez-Roj, RO Wright, AP Sanders, MJ Rosa
    Toxics, 2022-11-16;10(11):.
    Species: Human
    Sample Types: Serum
  4. CD14 and CD26 from serum exosomes are associated with type 2 diabetes, exosomal Cystatin C and CD14 are associated with metabolic syndrome and atherogenic index of plasma
    Authors: CP Pérez-Mace, E Flores-Alf, LDC Alarcón-Ro, A Vences-Vel, N Castro-Ala, E Martínez-M, M Ramirez
    PeerJ, 2022-07-12;10(0):e13656.
    Species: Human
    Sample Types: Cell Lysates
  5. Prenatal and early childhood critical windows for the association of nephrotoxic metal and metalloid mixtures with kidney function
    Authors: AP Sanders, C Gennings, M Tamayo-Ort, S Mistry, I Pantic, M Martinez, G Estrada-Gu, A Espejel-Nu, LT Olascoaga, RO Wright, MM Téllez-Roj, M Arora, C Austin
    Oncogene, 2022-06-27;166(0):107361.
    Species: Human
    Sample Types: Serum
  6. Acute kidney injury in hospitalized children with sickle cell anemia
    Authors: A Batte, S Menon, J Ssenkusu, S Kiguli, R Kalyesubul, J Lubega, EI Mutebi, RO Opoka, CC John, MC Starr, AL Conroy
    Bmc Nephrology, 2022-03-18;23(1):110.
    Species: Human
    Sample Types: Plasma
  7. Cerebrospinal Fluid EV Concentration and Size Are Altered in Alzheimer's Disease and Dementia with Lewy Bodies
    Authors: A Longobardi, R Nicsanu, S Bellini, R Squitti, M Catania, P Tiraboschi, C Saraceno, C Ferrari, R Zanardini, G Binetti, G Di Fede, L Benussi, R Ghidoni
    Cells, 2022-01-28;11(3):.
    Species: Human
    Sample Types: CSF
  8. Impact of Chronic Kidney Disease on the Associations of Cardiovascular Biomarkers With Adverse Outcomes in Patients With Suspected or Known Coronary Artery Disease: The EXCEED-J Study
    Authors: H Wada, T Shinozaki, M Suzuki, S Sakagami, Y Ajiro, J Funada, M Matsuda, M Shimizu, T Takenaka, Y Morita, K Yonezawa, H Matsubara, Y Ono, T Nakamura, K Fujimoto, A Ninomiya, T Kato, T Unoki, D Takagi, K Wada, M Wada, M Iguchi, H Yamakage, T Kusakabe, A Yasoda, A Shimatsu, K Kotani, N Satoh-Asah, M Abe, M Akao, K Hasegawa, EXCEED?J S
    Journal of the American Heart Association, 2022-01-20;11(3):e023464.
    Species: Human
    Sample Types: Serum
  9. Advanced glycation end products as predictors of renal function in youth with type 1 diabetes
    Authors: JM Forbes, S Le Bagge, S Righi, AK Fotheringh, LA Gallo, DA McCarthy, S Leung, T Baskervill, J Nisbett, A Morton, S Teasdale, N D'Silva, H Barrett, T Jones, J Couper, K Donaghue, N Isbel, DW Johnson, L Donnellan, P Deo, LK Akison, KM Moritz, T O'Moore-Su
    Scientific Reports, 2021-05-03;11(1):9422.
    Species: Human
    Sample Types: Serum
  10. Prenatal blood lead levels and reduced preadolescent glomerular filtration rate: Modification by body mass index
    Authors: C Saylor, M Tamayo-Ort, I Pantic, C Amarasiriw, N McRae, G Estrada-Gu, S Parra-Hern, MC Tolentino, AA Baccarelli, JJ Fadrowski, C Gennings, LM Satlin, RO Wright, MM Tellez-Roj, AP Sanders
    Environment international, 2021-03-04;0(0):106414.
    Species: Human
    Sample Types: Serum
  11. The impact of dyslipidemia on early markers of endothelial and renal dysfunction in children
    Authors: C Kosmeri, H Milionis, AP Vlahos, T Benekos, E Bairaktari, V Cholevas, E Siomou
    Journal of clinical lipidology, 2020-12-11;0(0):.
    Species: Human
    Sample Types: Serum
  12. Changes in Water Soluble Uremic Toxins and Urinary Acute Kidney Injury Biomarkers After 10- and 100-km Runs
    Authors: W Wo?yniec, K Kasprowicz, J Giebu?towi, N Korytowska, K Zorena, M Bartoszewi, P Rita-Tkach, M Renke, W Ratkowski
    Int J Environ Res Public Health, 2019-10-28;16(21):.
    Species: Human
    Sample Types: Urine
  13. The role of renal biomarkers to predict the need of surgery in congenital urinary tract obstruction in infants
    Authors: D Kostic, GPNS Beozzo, SB do Couto, AHT Kato, L Lima, P Palmeira, VLJ Krebs, V Bunduki, RPV Francisco, M Zugaib, FT Dénes, WB de Carvalh, VHK Koch
    J Pediatr Urol, 2019-03-16;0(0):.
    Species: Human
    Sample Types: Serum
  14. Evaluation of neutrophil gelatinase-associated lipocalin and cystatin C as biomarkers of acute kidney injury after ST-segment elevation myocardial infarction treated by percutaneous coronary intervention
    Authors: LS Nguyen, V Spagnoli, M Kerneis, M Hauguel-Mo, O Barthélémy, JP Collet, G Montalesco, J Silvain
    Arch Cardiovasc Dis, 2019-01-09;0(0):.
    Species: Human
    Sample Types: Plasma
  15. Increased Cathepsin S activity associated with decreased protease inhibitory capacity contributes to altered tear proteins in Sj�gren's Syndrome patients
    Authors: MC Edman, SR Janga, Z Meng, M Bechtold, AF Chen, C Kim, L Naman, A Sarma, N Teekappana, AY Kim, S Madrigal, S Singh, E Ortiz, S Christiana, DG Arkfeld, WJ Mack, M Heur, W Stohl, SF Hamm-Alvar
    Sci Rep, 2018-07-23;8(1):11044.
    Species: Human
    Sample Types: Tears
  16. Influence of the 6-month physical activity programs on renal function in obese boys
    Authors: I Lousa, H Nascimento, S Rocha, C Catarino, F Reis, C Rêgo, A Santos-Sil, A Seabra, S Ribeiro, L Belo
    Pediatr. Res., 2018-04-11;0(0):.
    Species: Human
    Sample Types: Plasma
  17. Association of Biomarker Clusters With Cardiac Phenotypes and Mortality in Patients With HIV Infection
    Authors: R Scherzer, SJ Shah, E Secemsky, J Butler, C Grunfeld, MG Shlipak, PY Hsue
    Circ Heart Fail, 2018-04-01;11(4):e004312.
    Species: Human
    Sample Types: Serum
  18. Identifying biomarkers of dementia prevalent among amnestic mild cognitively impaired ethnic female patients
    Alzheimers Res Ther, 2016-10-18;8(1):43.
    Species: Human
    Sample Types: Plasma
  19. Neonatal acute kidney injury following Valsartan exposure in utero: report of two cases
    Authors: V Drossou-Ag
    Hippokratia, 2016-01-01;20(1):73-75.
    Species: Human
    Sample Types: Urine
  20. Combined Biomarker Analysis for Risk of Acute Kidney Injury in Patients with ST-Segment Elevation Myocardial Infarction.
    Authors: Tung Y, Chang C, Chen Y, Chu P
    PLoS ONE, 2015-04-08;10(4):e0125282.
    Species: Human
    Sample Types: Serum
  21. A novel PKD1 variant demonstrates a disease-modifying role in trans with a truncating PKD1 mutation in patients with autosomal dominant polycystic kidney disease.
    Authors: Ali H, Hussain N, Naim M, Zayed M, Al-Mulla F, Kehinde E, Seaburg L, Sundsbak J, Harris P
    BMC Nephrol, 2015-03-01;16(0):26.
    Species: Human
    Sample Types: Serum
  22. Age-related changes of cystatin C expression and polarized secretion by retinal pigment epithelium: potential age-related macular degeneration links.
    Authors: Kay, Paul, Yang, Yit C, Hiscott, Paul, Gray, Donna, Maminishkis, Arvydas, Paraoan, Luminita
    Invest Ophthalmol Vis Sci, 2014-02-14;55(2):926-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Acute kidney injury biomarkers for patients in a coronary care unit: a prospective cohort study.
    Authors: Chen TH, Chang CH, Lin CY, Jenq CC, Chang MY, Tian YC, Hung CC, Fang JT, Yang CW, Wen MS, Lin FC, Chen YC
    PLoS ONE, 2012-02-22;7(2):e32328.
    Species: Human
    Sample Types: Serum
  24. Increased prevalence of albuminuria in HIV-infected adults with diabetes.
    Authors: Kim PS, Woods C, Dutcher L, Georgoff P, Rosenberg A, Mican JA, Kopp JB, Smith MA, Hadigan C
    PLoS ONE, 2011-09-13;6(9):e24610.
    Species: Human
    Sample Types: Serum
  25. Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
    Authors: Gunawardana CG, Kuk C, Smith CR
    J. Proteome Res., 2009-10-01;8(10):4705-13.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Use of cystatin C-based estimations of glomerular filtration rate in patients with type 2 diabetes.
    Authors: Chudleigh RA, Ollerton RL, Dunseath G
    Diabetologia, 2009-05-09;52(7):1274-8.
    Species: Human
    Sample Types: Serum
  27. A urinary biomarker profile for children with HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
    Kidney Int., 2009-04-08;76(2):207-14.
    Species: Human
    Sample Types: Urine
  28. Cystatin C in cerebrospinal fluid as a biomarker of ALS.
    Authors: Tsuji-Akimoto S, Yabe I, Niino M, Kikuchi S, Sasaki H
    Neurosci. Lett., 2009-03-06;452(1):52-5.
    Species: Human
    Sample Types: CSF
  29. Imbalanced serum concentration between cathepsin B and cystatin C in patients with pelvic inflammatory disease.
    Authors: Tsai HT, Wang PH, Tee YT, Lin LY, Hsieh YS, Yang SF
    Fertil. Steril., 2008-03-07;91(2):549-55.
    Species: Human
    Sample Types: Serum

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Reviews for Human Cystatin C Quantikine ELISA Kit

Average Rating: 4.7 (Based on 6 Reviews)

5 Star
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Human Cystatin C Quantikine ELISA Kit
By Anonymous on 08/19/2021
Sample Tested: Serum

Human Cystatin C Quantikine ELISA Kit
By Anonymous on 08/19/2021
Sample Tested: Serum,Urine

Human Cystatin C Quantikine ELISA Kit
By Anonymous on 05/26/2021
Sample Tested: Cell culture supernatant

Human Cystatin C Quantikine ELISA Kit
By Anonymous on 05/18/2021
Sample Tested: Cell culture supernatant,Human cell line

Human Cystatin C Quantikine ELISA Kit
By Anonymous on 11/27/2018
Sample Tested: Serum

Human Cystatin C Quantikine ELISA Kit
By Jessica Fernando on 12/04/2017
Sample Tested: Serum,Urine