Human Daxx Alexa Fluor® 532-conjugated Antibody

Catalog #: AF4548X Datasheet / COA / SDS

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AF4548X-100UG

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Human Daxx Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Daxx in Western blots. Detection of both reported Daxx isoforms is expected based on immunogen sequence.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Daxx
Met497-Gly626
Accession # Q9UER7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Daxx

Daxx (Fas death domain-associated-xx; also death domain-associated protein 6) is a ubiquitously expressed, proapoptotic protein that belongs to the Daxx family of molecules. Although it has a predicted MW of 81 kDa, it runs anomously at 110 kDa in SDS-PAGE. Daxx is found in the nucleus where it represses transcription. In the cytoplasm, it binds to ASK1, where it mediates Fas-induced apoptosis. Human Daxx is 740 amino acids (aa) in length and contains three coiled-coil regions (aa 180‑217; 358‑399; 430‑489), two NLSs, and an Asp/Glu-rich acidic region (aa 434‑572). One splice variant is known that shows a 15 aa substitution for aa 696‑740. Human Daxx shares 71% and 90% aa identity with mouse and dog Daxx, respectively.

Long Name

Fas Death Domain-associated Protein

Entrez Gene IDs

1616 (Human); 13163 (Mouse); 140926 (Rat)

Alternate Names

BING2; CENP-C binding protein; DAP6; DAP6MGC126245; Daxx; death domain-associated protein 6; death-associated protein 6; death-domain associated protein; EAP1; ETS1-associated protein 1; Fas death domain-associated protein; Fas-binding protein; hDaxx; MGC126246