Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Daxx Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Daxx
Met497-Gly626
Accession # Q9UER7

Specificity

Detects endogenous human Daxx in Western blots. Detection of both reported Daxx isoforms is expected based on immunogen sequence.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Daxx Antibody

Detection of Human Daxx antibody by Western Blot.

Detection of Human Daxx by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Daxx Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4548) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Daxx at approximately 115 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Applications for Human Daxx Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Daxx

Daxx (Fas death domain-associated-xx; also death domain-associated protein 6) is a ubiquitously expressed, proapoptotic protein that belongs to the Daxx family of molecules. Although it has a predicted MW of 81 kDa, it runs anomously at 110 kDa in SDS-PAGE. Daxx is found in the nucleus where it represses transcription. In the cytoplasm, it binds to ASK1, where it mediates Fas-induced apoptosis. Human Daxx is 740 amino acids (aa) in length and contains three coiled-coil regions (aa 180‑217; 358‑399; 430‑489), two NLSs, and an Asp/Glu-rich acidic region (aa 434‑572). One splice variant is known that shows a 15 aa substitution for aa 696‑740. Human Daxx shares 71% and 90% aa identity with mouse and dog Daxx, respectively.

Long Name

Fas Death Domain-associated Protein

Alternate Names

BING2, DAP6

Entrez Gene IDs

1616 (Human); 13163 (Mouse); 140926 (Rat)

Gene Symbol

DAXX

UniProt

Q9UER7

Additional Daxx Products

Product Documents for Human Daxx Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Daxx Antibody

For research use only

Related Research Areas

Apoptosis Adaptor Proteins

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