|Detection of Human Daxx by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Daxx Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4548) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Daxx at approximately 115 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.|
Daxx (Fas death domain-associated-xx; also death domain-associated protein 6) is a ubiquitously expressed, proapoptotic protein that belongs to the Daxx family of molecules. Although it has a predicted MW of 81 kDa, it runs anomously at 110 kDa in SDS-PAGE. Daxx is found in the nucleus where it represses transcription. In the cytoplasm, it binds to ASK1, where it mediates Fas-induced apoptosis. Human Daxx is 740 amino acids (aa) in length and contains three coiled-coil regions (aa 180‑217; 358‑399; 430‑489), two NLSs, and an Asp/Glu-rich acidic region (aa 434‑572). One splice variant is known that shows a 15 aa substitution for aa 696‑740. Human Daxx shares 71% and 90% aa identity with mouse and dog Daxx, respectively.
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