Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1621P
Human DC-SIGN+DC-SIGNR PE-conjugated Antibody in Data
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Product Details
Citations (2)
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Human DC-SIGN+DC-SIGNR PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes both human DC-SIGN and human DC-SIGNR on transfected cells. Does not react with parental mouse cells or irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 120612
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR
Accession # Q9H2X3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of DC‑SIGN+DC‑SIGNR in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN and DC-SIGNR by Flow Cytometry. View Larger

Detection of DC‑SIGN+DC‑SIGNR in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN and DC-SIGNR by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line transfected with (A) human DC-SIGN and (B) human DC-SIGNR was stained with Mouse Anti-Human DC-SIGN+DC-SIGNR PE-conjugated Monoclonal Antibody (Catalog # FAB1621P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-SIGN+DC-SIGNR

DC-SIGN (Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin) has been shown to play an important role in regulating Dendritic Cell (DC) and T cell interactions, including antigen presentation to T cells and enhancement of transinfection of CD4+ T cells by HIV-1 (1, 2). Efforts to identify additional type II membrane proteins resulted in the isolation of a molecule related in sequence to DC-SIGN known as DC-SIGNR (DC-SIGN Related) (3, 4). DC-SIGNR shares 73 - 80% amino acid homology with DC-SIGN and is located on human chromosome 19p13.3. Its structure is similar to DC-SIGN and therefore binds mannose residues in a calcium dependent fashion, including ICAM-3 and HIV-1 gp120 (5). DC-SIGNR, also known as L-SIGN (Liver/Lymph node-Specific ICAM-3-Grabbing Non-integrin) and DC‑SIGNR, is polymorphic since allelic variations of the exon 4 encoded sequence have been isolated (5). This is further supported by a study demonstrating the ability to isolate a large repertoire of DC-SIGNR transcripts largely the result of alternative splicing of the 7 coding exons (6). L-SIGN/DC-SIGNR is primarily transcribed in the liver and lymph nodes but not in monocyte derived DC (5). Expression of L-SIGN/DC-SIGNR is restricted to endothelial cells derived from liver sinusoids, lymph node sinuses and capillaries (7) although variable expression in placenta and some monocytic cell lines has also been reported, including both membrane and soluble isoforms of the protein (6). Expression of DC-SIGN is induced during the in vitro generation of DC from either monocytes or bone marrow progenitors, with maximal surface expression at day 7 of culture (1). Immature DC in the skin and mature DC in the tonsil have been demonstrated to express DC‑SIGN (8). Analysis of various tissues and cell lines suggests that DC-SIGN expression is restricted to DC (1) although a more recent report finds evidence of expression in placenta, resting monocytes and monocytic cell lines (6). This discrepancy may be partially related to the multiple isoforms of DC-SIGN transcripts, including both membrane and soluble forms, as well as exon splice variants reported in the latter study (6).

References
  1. Geijtenbeek, T.B.H. et al. (2000) Cell 100:575.
  2. Geijtenbeek, T.B.H. et al. (2000) Cell 100:587.
  3. Yokoyama-Kobayashi, M.T. et al. (1999) Gene 228:161.
  4. Soilleux, E.J. et al. (2000) J. Immunol. 165:2937.
  5. Bashirova, A.A. et al. (2001) J. Exp. Med. 193:671.
  6. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196..
  7. Pohlman, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
  8. Geijtenbeek, T.B.H. et al. (2000) Nature Immunol. 1:353.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin
Entrez Gene IDs
30835 (Human)
Alternate Names
DCSIGN+DCSIGNR; DC-SIGN+DC-SIGNR

Product Datasheets

Citations for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Severe fever with thrombocytopenia syndrome virus targets B cells in lethal human infections
    Authors: T Suzuki, Y Sato, K Sano, T Arashiro, H Katano, N Nakajima, M Shimojima, M Kataoka, K Takahashi, Y Wada, S Morikawa, S Fukushi, T Yoshikawa, M Saijo, H Hasegawa
    J. Clin. Invest., 2020;130(2):799-812.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Genome-wide small interfering RNA screens reveal VAMP3 as a novel host factor required for Uukuniemi virus late penetration.
    Authors: Meier R, Franceschini A, Horvath P, Tetard M, Mancini R, von Mering C, Helenius A, Lozach P
    J Virol, 2014;88(15):8565-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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