Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

  (1 citations)     
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Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Recognizes both human DC-SIGN and human DC-SIGNR on transfected cells. Does not react with parental mouse cells or irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2A Clone # 120612
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR
    Accession # Q9H2X3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of DC‑SIGN+DC‑SIGNR in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN and DC-SIGNR by Flow Cytometry. NIH‑3T3 mouse embryonic fibroblast cell line transfected with (A) human DC-SIGN and (B) human DC-SIGNR was stained with Mouse Anti-Human DC‑SIGN+DC‑SIGNR PE‑conjugated Monoclonal Antibody (Catalog # FAB1621P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DC-SIGN+DC-SIGNR
DC-SIGN (Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin) has been shown to play an important role in regulating Dendritic Cell (DC) and T cell interactions, including antigen presentation to T cells and enhancement of transinfection of CD4+ T cells by HIV-1 (1, 2). Efforts to identify additional type II membrane proteins resulted in the isolation of a molecule related in sequence to DC-SIGN known as DC-SIGNR (DC-SIGN Related) (3, 4). DC-SIGNR shares 73 - 80% amino acid homology with DC-SIGN and is located on human chromosome 19p13.3. Its structure is similar to DC-SIGN and therefore binds mannose residues in a calcium dependent fashion, including ICAM-3 and HIV-1 gp120 (5). DC-SIGNR, also known as L-SIGN (Liver/Lymph node-Specific ICAM-3-Grabbing Non-integrin) and DC‑SIGNR, is polymorphic since allelic variations of the exon 4 encoded sequence have been isolated (5). This is further supported by a study demonstrating the ability to isolate a large repertoire of DC-SIGNR transcripts largely the result of alternative splicing of the 7 coding exons (6). L-SIGN/DC-SIGNR is primarily transcribed in the liver and lymph nodes but not in monocyte derived DC (5). Expression of L-SIGN/DC-SIGNR is restricted to endothelial cells derived from liver sinusoids, lymph node sinuses and capillaries (7) although variable expression in placenta and some monocytic cell lines has also been reported, including both membrane and soluble isoforms of the protein (6). Expression of DC-SIGN is induced during the in vitro generation of DC from either monocytes or bone marrow progenitors, with maximal surface expression at day 7 of culture (1). Immature DC in the skin and mature DC in the tonsil have been demonstrated to express DC‑SIGN (8). Analysis of various tissues and cell lines suggests that DC-SIGN expression is restricted to DC (1) although a more recent report finds evidence of expression in placenta, resting monocytes and monocytic cell lines (6). This discrepancy may be partially related to the multiple isoforms of DC-SIGN transcripts, including both membrane and soluble forms, as well as exon splice variants reported in the latter study (6).
  • References:
    1. Geijtenbeek, T.B.H. et al. (2000) Cell 100:575.
    2. Geijtenbeek, T.B.H. et al. (2000) Cell 100:587.
    3. Yokoyama-Kobayashi, M.T. et al. (1999) Gene 228:161.
    4. Soilleux, E.J. et al. (2000) J. Immunol. 165:2937.
    5. Bashirova, A.A. et al. (2001) J. Exp. Med. 193:671.
    6. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196..
    7. Pohlman, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
    8. Geijtenbeek, T.B.H. et al. (2000) Nature Immunol. 1:353.
  • Long Name:
    Dendritic Cell-specific ICAM-3-grabbing Non-integrin
  • Entrez Gene IDs:
    30835 (Human)
  • Alternate Names:
    DCSIGN+DCSIGNR; DC-SIGN+DC-SIGNR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Genome-wide small interfering RNA screens reveal VAMP3 as a novel host factor required for Uukuniemi virus late penetration.
    Authors: Meier R, Franceschini A, Horvath P, Tetard M, Mancini R, von Mering C, Helenius A, Lozach P
    J Virol, 2014;88(15):8565-78.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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Isotype Controls
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Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 14  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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