Human DC-SIGNR/CD299 Alexa Fluor® 700-conjugated Antibody

FAB162N Datasheet / COA / SDS

Clone 120604 was used by HLDA to establish CD designation

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FAB162N-100UG

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Human DC-SIGNR/CD299 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DC‑SIGNR/CD299.

Source

Monoclonal Mouse IgG2b Clone # 120604

Immunogen

NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Accession # Q9H2X3

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

Human DC‑SIGNR/CD299 transfected 3T3 mouse embryonic fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DC-SIGNR/CD299

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

References

Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
Pohlmann, S. et al. (2003) J. Virol. 77:4070.
Simmons, L.G. et al. (2003) J. Virol. 77:1337.
Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
Feinberg, H. et al. (2001) Science 294:2163.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2

Entrez Gene IDs

10332 (Human)

Alternate Names

CD209L; CD209L1; CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2; CD299 antigenMGC129964; CD299; CLEC4M; C-type lectin domain family 4, member M; DC-SIGN2; DC-SIGN2CD209 antigen-like protein 1; DCSIGNR; DC-SIGNR; DC-SIGNRDC-SIGN-related protein; DCSIGNRMGC47866; Dendritic cell-specific ICAM-3-grabbing non-integrin 2; HP10347; liver/lymph node-specific ICAM-3 grabbing non-integrin; Liver/lymph node-specific ICAM-3-grabbing non-integrin; LSIGN; L-SIGN; LSIGNC-type lectin domain family 4 member M; mannose binding C-type lectin DC-SIGNR