Protein A or G purified from hybridoma culture supernatant
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299 Accession # Q9H2X3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
The adhesion of NIH-3T3 mouse embryonic fibroblast cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 2.5 µg/mL of the antibody.
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of DC‑SIGNR/CD299 in Human DC‑SIGNR/CD299 Transfected 3T3 Mouse Cell Line by Flow Cytometry.
Human DC‑SIGNR/CD299 and DC‑SIGN transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGNR/CD299 Monoclonal Antibody (Catalog # MAB162, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).
Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
Pohlmann, S. et al. (2003) J. Virol. 77:4070.
Simmons, L.G. et al. (2003) J. Virol. 77:1337.
Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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