Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).
Human DC-SIGNR/CD299 Alexa Fluor® 647-conjugated Antibody
R&D Systems | Catalog # FAB162R
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Accession # Q9H2X3
Specificity
Clonality
Host
Isotype
Applications for Human DC-SIGNR/CD299 Alexa Fluor® 647-conjugated Antibody
Flow Cytometry
Sample: Human DC‑SIGNR/CD299 transfected 3T3 mouse embryonic fibroblast cell line
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Background: DC-SIGNR/CD299
References
- Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
- Pohlmann, S. et al. (2003) J. Virol. 77:4070.
- Simmons, L.G. et al. (2003) J. Virol. 77:1337.
- Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
- Feinberg, H. et al. (2001) Science 294:2163.
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Product Documents for Human DC-SIGNR/CD299 Alexa Fluor® 647-conjugated Antibody
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Product Specific Notices for Human DC-SIGNR/CD299 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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