Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 120604
View all DC-SIGNR/CD299 Primary Antibodies »

Product Specifications

Immunogen

NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Accession # Q9H2X3

Specificity

Detects human DC‑SIGNR/CD299.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DC‑SIGNR/CD299 PE‑conjugated Antibody

Detection of DC‑SIGNR/CD299 antibody in 3T3 Mouse Cell Line Transfected with Human DC-SIGNR/CD299 antibody by Flow Cytometry.

Detection of DC‑SIGNR/CD299 in 3T3 Mouse Cell Line Transfected with Human DC-SIGNR/CD299 by Flow Cytometry.

3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR/CD299 was stained with Mouse Anti-Human DC-SIGNR/CD299 PE-conjugated Monoclonal Antibody (Catalog # FAB162P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of DC-SIGNR/CD299 by Flow Cytometry

Detection of DC-SIGNR/CD299 by Flow Cytometry

Recombinant MCP-1 protein in DENV replication. (A) Flow cytometry was used to detect the dengue virus envelope protein (E) and L-SIGN expression on the cell surface in DENV-infected SK-HEP-1 pcDNA3.1 and SK-HEP-1 pcDNA3.1 L-SIGN with 0, 0.5, and 5 ng/mL MCP-1. (B) DENV copy numbers were counted by fluorescence intensity in DENV-infected SK-HEP-1 pcDNA3.1 and SK-HEP-1 pcDNA3.1 L-SIGN with 0, 0.5, and 5 ng/mL MCP-1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40143272), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human DC‑SIGNR/CD299 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: 3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR/CD299

Reviewed Applications

Read 1 review rated 5 using FAB162P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-SIGNR/CD299

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1-4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

References

  1. Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
  2. Pohlmann, S. et al. (2003) J. Virol. 77:4070.
  3. Simmons, L.G. et al. (2003) J. Virol. 77:1337.
  4. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
  5. Feinberg, H. et al. (2001) Science 294:2163.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2

Alternate Names

CD209L, CD299, CLEC4M, DC-SIGN2, DCSIGNR, LSIGN

Entrez Gene IDs

10332 (Human)

Gene Symbol

CLEC4M

UniProt

Q9H2X3

Additional DC-SIGNR/CD299 Products

Product Documents for Human DC‑SIGNR/CD299 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DC‑SIGNR/CD299 PE‑conjugated Antibody

For research use only

Citations for Human DC‑SIGNR/CD299 PE‑conjugated Antibody

Customer Reviews for Human DC‑SIGNR/CD299 PE‑conjugated Antibody (1)

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Customer Images

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  • Human DC-SIGNR/CD299 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peritoneal macrophages
    Species: Human
    Verified Customer | Posted 08/12/2024
    Expression of DC-SIGNR on human monocyte-derived macrophages. The cells were treated with 10 μg/ml of the DC-SIGNR PE antibody (catalogue # FAB162P) (blue) or mouse IgG2b PE isotype control antibody (Red)
    Human DC‑SIGNR/CD299 PE‑conjugated Antibody FAB162P

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