Human DCIR/CLEC4A Alexa Fluor® 488-conjugated Antibody

FAB1748G Datasheet / COA / SDS

Clone 216110 was used by HLDA to establish CD designation

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FAB1748G-100UG

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Detection of DCIR/CLEC4A in Human Blood Neutrophils by Flow Cytometry.

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Human DCIR/CLEC4A Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DCIR in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 216110

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DCIR isoform 1
Gln70-Leu237
Accession # Q9UMR7

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of DCIR/CLEC4A in Human Blood Neutrophils by Flow Cytometry. Human peripheral blood neutrophils were stained with Mouse Anti-Human DCIR/CLEC4A Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1748G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: DCIR/CLEC4A

DCIR, also known as Lectin-like Immunoreceptor (LLIR), is a type II membrane protein belonging to the C-type lectin domain family and is designated CLEC4A (previously designated CLECSF6). Four transcript variants encoding distinct isoforms have been identified. DCIR contains one carbohydrate recognition domain in its C-terminal extracellular domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. Besides DC, DCIR is expressed on B cells, monocytes/macrophages and granulocytes.

Long Name

Dendritic Cell Immunoreceptor

Entrez Gene IDs

50856 (Human); 26888 (Mouse)

Alternate Names

CD367; CLEC4A; CLECSF6; CLECSF6C-type lectin superfamily member 6; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 6; C-type lectin domain family 4 member A; C-type lectin domain family 4, member A; DCIR; DCIRLLIR; DDB27; DDB27C-type lectin DDB27; Dendritic cell immunoreceptor; Lectin-like immunoreceptor; LLIR