Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Cross-linking

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 216110
View all DCIR/CLEC4A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DCIR isoform 1
Gln70-Leu237
Accession # Q9UMR7

Specificity

Detects human DCIR in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human DCIR/CLEC4A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood neutrophils

Western Blot

1 µg/mL
Sample: Recombinant Human DCIR/CLEC4A
under non-reducing conditions only

Reviewed Applications

Read 1 review rated 4 using MAB1748 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DCIR/CLEC4A

DCIR, also known as Lectin-like Immunoreceptor (LLIR), is a type II membrane protein belonging to the C-type lectin domain family and is designated CLEC4A (previously designated CLECSF6). Four transcript variants encoding distinct isoforms have been identified. DCIR contains one carbohydrate recognition domain in its C-terminal extracellular domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. Besides DC, DCIR is expressed on B cells, monocytes/macrophages and granulocytes.

Long Name

Dendritic Cell Immunoreceptor

Alternate Names

CD367, CLEC4A, CLECSF6, DDB27, LLIR

Entrez Gene IDs

50856 (Human); 26888 (Mouse)

Gene Symbol

CLEC4A

UniProt

Q9UMR7

Additional DCIR/CLEC4A Products

Product Documents for Human DCIR/CLEC4A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DCIR/CLEC4A Antibody

For research use only

Citations for Human DCIR/CLEC4A Antibody

Customer Reviews for Human DCIR/CLEC4A Antibody (1)

4 out of 5
1 Customer Rating
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  • Human DCIR/CLEC4A Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 07/27/2016

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