Human DCIR/CLEC4A APC-conjugated Antibody

Clone 216110 was used by HLDA to establish CD designation
(1 Review)
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human DCIR/CLEC4A in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 216110
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human DCIR isoform 1
    Gln70-Leu237
    Accession # Q9UMR7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of DCIR/CLEC4A in Human Blood Neutrophils by Flow Cytometry.

Human peripheral blood neutrophils were stained with Mouse Anti-Human DCIR/CLEC4A APC‑conjugated Monoclonal Antibody (Catalog # FAB1748A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DCIR/CLEC4A
DCIR, also known as Lectin-like Immunoreceptor (LLIR), is a type II membrane protein belonging to the C-type lectin domain family and is designated CLEC4A (previously designated CLECSF6). Four transcript variants encoding distinct isoforms have been identified. DCIR contains one carbohydrate recognition domain in its C-terminal extracellular domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. Besides DC, DCIR is expressed on B cells, monocytes/macrophages and granulocytes.
  • Long Name:
    Dendritic Cell Immunoreceptor
  • Entrez Gene IDs:
    50856 (Human); 26888 (Mouse)
  • Alternate Names:
    CD367; CLEC4A; CLECSF6; CLECSF6C-type lectin superfamily member 6; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 6; C-type lectin domain family 4 member A; C-type lectin domain family 4, member A; DCIR; DCIRLLIR; DDB27; DDB27C-type lectin DDB27; Dendritic cell immunoreceptor; Lectin-like immunoreceptor; LLIR
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 11  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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Read what people are saying who have used Human DCIR/CLEC4A APC-conjugated Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Human Anonymous 08/08/2016
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Summary

Application Flow Cytometry
Sample Tested Dendritic cells
Species Human

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