Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 143-41
View all DEP-1/CD148 Primary Antibodies »

Product Specifications

Immunogen

Phytohemagglutinin-stimulated human peripheral blood mononuclear cells

Specificity

Detects human DEP-1/CD148 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human DEP-1/CD148 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human peripheral blood lymphocytes

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T‑cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

References

  1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
  2. Sorby, M. et al. (2001) Oncogene 20:5219.
  3. Jandt, E. et al. (2003) Oncogene 22:4175.
  4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
  5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
  6. Borges, L.G. et al. (1996) Circ. Res. 79:570.
  7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
  8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
  9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
  10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
  11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
  12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.

Long Name

Density Enhanced Receptor Protein Tyrosine Phosphatase

Alternate Names

CD148, DEP1, HPTP-eta, PTPRJ

Entrez Gene IDs

5795 (Human); 19271 (Mouse); 29645 (Rat)

Gene Symbol

PTPRJ

Additional DEP-1/CD148 Products

Product Documents for Human DEP-1/CD148 Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human DEP-1/CD148 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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