Human DEP-1/CD148 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human DEP-1/CD148 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 143-41
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Phytohemagglutinin-stimulated human peripheral blood mononuclear cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood lymphocytes
  • Immunoprecipitation
    2.5 µg/500 µg cell lysate
    HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human DEP‑1/CD148 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human DEP‑1/CD148 Monoclonal Antibody (Catalog # MAB1934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEP‑1/CD148 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T‑cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

  • References:
    1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
    2. Sorby, M. et al. (2001) Oncogene 20:5219.
    3. Jandt, E. et al. (2003) Oncogene 22:4175.
    4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
    5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
    6. Borges, L.G. et al. (1996) Circ. Res. 79:570.
    7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
    8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
    9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
    10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
    11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
    12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.
  • Long Name:
    Density Enhanced Receptor Protein Tyrosine Phosphatase
  • Entrez Gene IDs:
    5795 (Human); 19271 (Mouse); 29645 (Rat)
  • Alternate Names:
    CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Species
Applications
Sample Type
  1. A novel splice variant of the protein tyrosine phosphatase PTPRJ that encodes for a soluble protein involved in angiogenesis
    Authors: A Bilotta, V Dattilo, S D'Agostino, S Belviso, S Scalise, M Bilotta, E Gaudio, F Paduano, N Perrotti, T Florio, A Fusco, R Iuliano, F Trapasso
    Oncotarget, 2016;0(0):.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. The protein tyrosine phosphatase DEP-1/PTPRJ promotes breast cancer cell invasion and metastasis.
    Authors: Spring K, Fournier P, Lapointe L, Chabot C, Roussy J, Pommey S, Stagg J, Royal I
    Oncogene, 2015;34(44):5536-47.
  3. Phosphorylation of DEP-1/PTPRJ on threonine 1318 regulates Src activation and endothelial cell permeability induced by vascular endothelial growth factor.
    Authors: Spring K, Lapointe L, Caron C, Langlois S, Royal I
    Cell Signal, 2014;26(6):1283-93.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2011;134(2):167-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG1 Isotype Control

Ctrl MAB002R 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human DEP-1/CD148 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information