Human DEP-1/CD148 Alexa Fluor® 488-conjugated Antibody

FAB1934G Datasheet / COA / SDS

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FAB1934G-100UG

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Human DEP-1/CD148 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DEP-1/CD148 in Western blots.

Source

Monoclonal Mouse IgG1 Clone # 143-41

Immunogen

Phytohemagglutinin-stimulated human peripheral blood mononuclear cells

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

Human peripheral blood lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T‑cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

References

Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
Sorby, M. et al. (2001) Oncogene 20:5219.
Jandt, E. et al. (2003) Oncogene 22:4175.
Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
Borges, L.G. et al. (1996) Circ. Res. 79:570.
de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
Holsinger, L.J. et al. (2002) Oncogene 21:7067.

Long Name

Density Enhanced Receptor Protein Tyrosine Phosphatase

Entrez Gene IDs

5795 (Human); 19271 (Mouse); 29645 (Rat)

Alternate Names

CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of