Detection of DEP‑1/CD148 in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human DEP‑1/CD148 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1934F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP Receptor Type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-Catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.
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Density Enhanced Receptor Protein Tyrosine Phosphatase
Entrez Gene IDs:
5795 (Human); 19271 (Mouse); 29645 (Rat)
CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 184.108.40.206; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of
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