Human DEP-1/CD148 Fluorescein-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human DEP-1/CD148 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 143-41
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Phytohemagglutinin-stimulated human peripheral blood mononuclear cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of DEP‑1/CD148 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human DEP‑1/CD148 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1934F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP Receptor Type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-Catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

  • References:
    1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
    2. Sorby, M. et al. (2001) Oncogene 20:5219.
    3. Jandt, E. et al. (2003) Oncogene 22:4175.
    4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
    5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
    6. Borges, L.G. et al. (1996) Circ. Res. 79:570.
    7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
    8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
    9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
    10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
    11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
    12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.
  • Long Name:
    Density Enhanced Receptor Protein Tyrosine Phosphatase
  • Entrez Gene IDs:
    5795 (Human); 19271 (Mouse); 29645 (Rat)
  • Alternate Names:
    CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Polymorphisms of protein tyrosine phosphatase CD148 influence Fc gamma RIIA-dependent platelet activation and the risk of heparin-induced thrombocytopenia.
    Authors: Rollin J, Pouplard C, Gratacap MP, Leroux D, May MA, Aupart M, Gouilleux-Gruart V, Payrastre B, Gruel Y
    Blood, 2012;120(6):1309-16.
    Species: Human
    Sample Type: Whole Cells
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 9  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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