Human Dihydrolipoamide Dehydrogenase/DLD Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # FAB8646AFP647

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1016619
View all Dihydrolipoamide Dehydrogenase/DLD Primary Antibodies »

Product Specifications

Specificity

Detects human Dihydrolipoamide Dehydrogenase/DLD in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Dihydrolipoamide Dehydrogenase/DLD

Dihydrolipoamide dehydrogenase (DLD), also known as LADH, is an NAD-dependent oxidoreductase in the mitochondrial matrix (1). DLD serves as the E3 subunit of four mitochondrial enzyme complexes: pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, branched chain alpha-ketotacid dehydrogenase, and the glycine cleavage system (2, 3). It is active as a 120 kDa dimer that catalyzes oxidation within these enzyme complexes. Several mutations of human DLD have been described, some of which contribute to the loss of respiratory function during oxidative stress (4, 5). DLD mutations located at the interface between dimer subunits can impair dimer formation (5, 6). The involvement of DLD in the regulation of lipid peroxidation and lactate metabolism is important for mouse hippocampal neuroblast proliferation and hamster sperm capacitation, respectively (7, 8). DLD polymorphisms in insects can increase their resistance to the pesticide phosphine gas, while they can increase the sensitivity to arsenite in the nematode C. elegans (9). Mature human DLD shares 95-96% amino acid (aa) sequence identity with hamster, mouse, and rat DLD. Alternative splicing generates additional human DLD isoforms that lack the N-terminal 99 aa or carry a deletion of aa 147-194.

References

  1. Brand, M.D. (2010) Exp. Gerontol. 45:466.
  2. Johnson, M.T. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14512.
  3. Sundquist, A.R. and R.C. Fahey (1988) J. Bacteriol. 170:3459.
  4. Ambrus, A. et al. (2011) Hum. Mol. Genet. 20:2984.
  5. Vaubel, R.A. et al. (2011) J. Biol. Chem. 286:40232.
  6. Babady, N.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:6158.
  7. Calingasan, N.Y. et al. (2008) Neuroscience 153:986.
  8. Panneerdoss, S. et al. (2012) J. Androl. 33:699.
  9. Schlipalius, D.I. et al. (2012) Science 338:807.

Alternate Names

Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3

Entrez Gene IDs

1738 (Human); 13382 (Mouse); 298942 (Rat)

Gene Symbol

DLD

UniProt

P09622

Additional Dihydrolipoamide Dehydrogenase/DLD Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Redox Enzymes

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