E-Cadherin/Cadherin-1 (ECAD) Is a transmembrane cell adhesion protein. It forms homophilic clusters between epithelial cells and is a critical component of adherens junctions. These junctions are critical for the maintenance of the epithelial barrier and epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition (EMT) and cell migration, and E-Cadherin loss contributes to cancer metastasis. The shed ECD is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation.
Human E-Cadherin Quantikine ELISA Kit
R&D Systems | Catalog # DCADE0B
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Key Product Details
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL)
Sensitivity
0.126 ng/mL
Assay Range
0.625-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
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Product Summary for Human E-Cadherin Quantikine ELISA Kit
The Quantikine™ Human E-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human E-Cadherin in cell culture supernates, serum, plasma, saliva, and urine. It contains HEK293-expressed recombinant human E-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human E-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human E-Cadherin.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human E-Cadherin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
| Sample Type | Mean (ng/mL) | Range (ng/mL) |
| Serum (n=30) | 250 | 127-492 |
| EDTA Plasma (n=30) | 242 | 131-479 |
| Heparin Plasma (n=30) | 236 | 116-479 |
| Urine (n=10) | 60.6 | 3.03-121 |
| Saliva (n=10) | 145 | 21.4-278 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 2.42 | 7.06 | 14.6 | 2.19 | 6.55 | 14.2 |
| Standard Deviation | 0.047 | 0.145 | 0.266 | 0.136 | 0.368 | 0.845 |
| CV% | 1.9 | 2.1 | 1.8 | 6.2 | 5.6 | 6.0 |
Recovery for Human E-Cadherin Quantikine ELISA Kit
The recovery of human E-Cadherin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=30) | 242 | 131-479 |
| Heparin Plasma (n=30) | 236 | 116-479 |
| Saliva (n=10) | 145 | 21.4-278 |
| Serum (n=30) | 250 | 127-492 |
| Urine (n=10) | 60 | 3.03-121 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human E-Cadherin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human E-Cadherin Quantikine ELISA Kit
Human E-Cadherin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: E-Cadherin
Alternate Names
Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin
Gene Symbol
CDH1
Additional E-Cadherin Products
Product Documents for Human E-Cadherin Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human E-Cadherin Quantikine ELISA Kit
For research use only
Citations for Human E-Cadherin Quantikine ELISA Kit
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
