Detects human EDA/Ectodysplasin (EDA-A1 and EDA-A2) in ELISAs. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse EDA is observed, and no cross-reactivity with recombinant human (rh) TNF-alpha, rhXEDAR, or rhEDAR is observed.
Monoclonal Mouse IgG2B Clone # 174817
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human EDA-A2/Ectodysplasin A2 Ala179-Ser389 (predicted) Accession # NP_001005609
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Human EDA-A2/Ectodysplasin A2 Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Human EDA/Ectodysplasin Antibody (Catalog # MAB9222)
ELISA Detection (Matched Antibody Pair)
Human EDA/Ectodysplasin Biotinylated Antibody (Catalog # BAM9221)
Recombinant Human EDA-A2 Protein (Catalog # 922-ED)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The products of the Ectodysplasin A gene (EDA, also called Tabby) are type II transmembrane proteins that belong to the TNF superfamily. The EDA gene encodes two splice variants, designated EDA-A1 and EDA-A2, that differ only by the deletion of two amino acids in the extracellular domain of EDA-A2. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. The extracellular domains of human and mouse EDA-A2 share approximately 94% amino acid sequence identity.
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