Human EDA/Ectodysplasin Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human EDA/Ectodysplasin (EDA-A1 and EDA-A2) in ELISAs. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse EDA is observed, and no cross-reactivity with recombinant human (rh) TNF-alpha, rhXEDAR, or rhEDAR is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 174817
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human EDA-A2/Ectodysplasin A2
    Ala179-Ser389 (predicted)
    Accession # NP_001005609
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
    • Human EDA-A2/Ectodysplasin A2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human EDA/Ectodysplasin Antibody (Catalog # MAB9222)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human EDA/Ectodysplasin Biotinylated Antibody (Catalog # BAM9221)
  • ELISA Standard
     
    Recombinant Human EDA-A2 Protein (Catalog # 922-ED)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EDA/Ectodysplasin

The products of the Ectodysplasin A gene (EDA, also called Tabby) are type II transmembrane proteins that belong to the TNF superfamily. The EDA gene encodes two splice variants, designated EDA-A1 and EDA-A2, that differ only by the deletion of two amino acids in the extracellular domain of EDA-A2. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. The extracellular domains of human and mouse EDA-A2 share approximately 94% amino acid sequence identity.

  • Entrez Gene IDs:
    1896 (Human); 13607 (Mouse)
  • Alternate Names:
    ectodysplasin A; ED1; EDA; Tabby; XHED; XLHED
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