eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is an important member of the translation initiation mechanism. It serves as a scaffold for other initiation factors needed for the recruitment of mRNA to the ribosome, including eIF4E, eIF4A, eIF3, and PABP (poly (A)-binding protein). The eIF4E/eIF4G interaction binds to the 5’ m7GTP mRNA cap, while the PABP/eIF4G interaction binds to the poly (A) tail of mRNA. eIF4A is an ATP-dependent RNA helicase. These interactions link the process of poly (A) binding, mRNA cap recognition, and unwinding of secondary structure. In addition when eIF4G is cleaved by a viral protease the majority of host cell mediated translation is diverted to an IRES (internal ribosomal entry site) mediated initiation of translation.
Discontinued Product
AF4018 has been discontinued.
View all eIF4G1 products.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human eIF4G1
Leu1401-Asn1599
Accession # Q04637
Leu1401-Asn1599
Accession # Q04637
Specificity
Detects human eIF4G1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human eIF4G1 Antibody
Detection of Human eIF4G by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human eIF4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for eIF4G at approximately 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human eIF4G1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: eIF4G1
Long Name
Eukaryotic Translation Initiation Factor 4 gamma 1
Alternate Names
DKFZp686A1451, EIF4F, eIF-4G 1, eIF-4G1, EIF4-gamma, eIF-4-gamma 1, EIF4GI, EIF4Gp220, eucaryotic translation initiation factor 4G, eukaryotic translation initiation factor 4 gamma 1, eukaryotic translation initiation factor 4 gamma, 1, P220
Entrez Gene IDs
1981 (Human)
Gene Symbol
EIF4G1
UniProt
Additional eIF4G1 Products
Product Documents for Human eIF4G1 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human eIF4G1 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
