|Detection of Human eIF4G by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human eIF4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for eIF4G at approximately 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.|
eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is an important member of the translation initiation mechanism. It serves as a scaffold for other initiation factors needed for the recruitment of mRNA to the ribosome, including eIF4E, eIF4A, eIF3, and PABP (poly (A)-binding protein). The eIF4E/eIF4G interaction binds to the 5’ m7GTP mRNA cap, while the PABP/eIF4G interaction binds to the poly (A) tail of mRNA. eIF4A is an ATP-dependent RNA helicase. These interactions link the process of poly (A) binding, mRNA cap recognition, and unwinding of secondary structure. In addition when eIF4G is cleaved by a viral protease the majority of host cell mediated translation is diverted to an IRES (internal ribosomal entry site) mediated initiation of translation.
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