Human eIF4G1 Antibody

R&D Systems | Catalog # AF4018

R&D Systems
Discontinued Product
AF4018 has been discontinued. View all eIF4G1 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all eIF4G1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human eIF4G1
Leu1401-Asn1599
Accession # Q04637

Specificity

Detects human eIF4G1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human eIF4G1 Antibody

Detection of Human eIF4G antibody by Western Blot.

Detection of Human eIF4G by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human eIF4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for eIF4G at approximately 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human eIF4G1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: eIF4G1

eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is an important member of the translation initiation mechanism. It serves as a scaffold for other initiation factors needed for the recruitment of mRNA to the ribosome, including eIF4E, eIF4A, eIF3, and PABP (poly (A)-binding protein). The eIF4E/eIF4G interaction binds to the 5’ m7GTP mRNA cap, while the PABP/eIF4G interaction binds to the poly (A) tail of mRNA. eIF4A is an ATP-dependent RNA helicase. These interactions link the process of poly (A) binding, mRNA cap recognition, and unwinding of secondary structure. In addition when eIF4G is cleaved by a viral protease the majority of host cell mediated translation is diverted to an IRES (internal ribosomal entry site) mediated initiation of translation.

Long Name

Eukaryotic Translation Initiation Factor 4 gamma 1

Alternate Names

DKFZp686A1451, EIF4F, eIF-4G 1, eIF-4G1, EIF4-gamma, eIF-4-gamma 1, EIF4GI, EIF4Gp220, eucaryotic translation initiation factor 4G, eukaryotic translation initiation factor 4 gamma 1, eukaryotic translation initiation factor 4 gamma, 1, P220

Entrez Gene IDs

1981 (Human)

Gene Symbol

EIF4G1

UniProt

Q04637

Additional eIF4G1 Products

Product Documents for Human eIF4G1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human eIF4G1 Antibody

For research use only

Related Research Areas

Translation Regulation

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