Human eIF4G1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human eIF4G by Western Blot.
1 Image
Product Details
Supplemental Products

Human eIF4G1 Antibody Summary

Species Reactivity
Detects human eIF4G1 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human eIF4G1
Accession # Q04637
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human eIF4G antibody by Western Blot. View Larger

Detection of Human eIF4G by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human eIF4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for eIF4G at approximately 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eIF4G1

eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is an important member of the translation initiation mechanism. It serves as a scaffold for other initiation factors needed for the recruitment of mRNA to the ribosome, including eIF4E, eIF4A, eIF3, and PABP (poly (A)-binding protein). The eIF4E/eIF4G interaction binds to the 5’ m7GTP mRNA cap, while the PABP/eIF4G interaction binds to the poly (A) tail of mRNA. eIF4A is an ATP-dependent RNA helicase. These interactions link the process of poly (A) binding, mRNA cap recognition, and unwinding of secondary structure. In addition when eIF4G is cleaved by a viral protease the majority of host cell mediated translation is diverted to an IRES (internal ribosomal entry site) mediated initiation of translation.

Long Name
Eukaryotic Translation Initiation Factor 4 gamma 1
Entrez Gene IDs
1981 (Human)
Alternate Names
DKFZp686A1451; EIF4F; eIF-4G 1; eIF4G1; EIF-4G1; eIF-4-gamma 1; EIF4-gamma; EIF4GI; EIF4Gp220; eucaryotic translation initiation factor 4G; eukaryotic translation initiation factor 4 gamma 1; eukaryotic translation initiation factor 4 gamma, 1; P220

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


Reviews for Human eIF4G1 Antibody

There are currently no reviews for this product. Be the first to review Human eIF4G1 Antibody and earn rewards!

Have you used Human eIF4G1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review