Detects human EOMES in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human (rh) Brachyury, rhEOMES (aa 1-115), recombinant mouse
EOMES (aa 1-126), rhTBX2, 3, 5, 6, 18, or 20 is observed.
Monoclonal Mouse IgG2B Clone # 644730
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human EOMES Gly471-Pro686 Accession # O95936
Supplied in a saline solution containing BSA and Sodium Azide.
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of EOMES in Mesendoderm-differentiated BG01V Human Cells by Flow Cytometry. Mesendoderm-differentiated BG01V human embryonic stem cells were stained with Mouse Anti-Human EOMES APC‑conjugated Monoclonal Antibody (Catalog # IC6166A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
EOMES (Eomesodermin; Eo from Greek meaning “dawn”/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage.
Entrez Gene IDs:
8320 (Human); 13813 (Mouse); 316052 (Rat)
eomesodermin (Xenopus laevis) homolog; Eomesodermin; t box, brain, 2; T-box brain protein 2; T-box brain2; TBR2; TBR-2; TBR2eomesodermin homolog; T-brain-2
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