Human ErbB3/Her3 APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human ErbB3/Her3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human EGF R is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 66223
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
    Accession # P21860
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ErbB3/Her3 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ErbB3/Her3 APC‑conjugated Monoclonal Antibody (Catalog # FAB3481A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ErbB3/Her3

ErbB3, also known as Her3 (Human Epidermal Growth Factor Receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the Epidermal Growth Factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the Heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of Phosphoinositide 3-Kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.

  • References:
    1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
    2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
    3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
    4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
    5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
    6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
    7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
    8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
    9. Schlessinger, J. (2000) Cell 103:211.
    10. Daly, R.J. (1999) Growth Factors 16:255.
  • Long Name:
    Receptor Tyrosine Protein Kinase ErbB3
  • Entrez Gene IDs:
    2065 (Human); 13867 (Mouse)
  • Alternate Names:
    c-erbB3; EC 2.7.10; EC; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Growth-factor-driven rescue to receptor tyrosine kinase (RTK) inhibitors through Akt and Erk phosphorylation in pediatric low grade astrocytoma and ependymoma.
    Authors: Sie M, den Dunnen W, Lourens H, Meeuwsen-de Boer T, Scherpen F, Zomerman W, Kampen K, Hoving E, de Bont E
    PLoS ONE, 2015;10(3):e0122555.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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