Human ErbB3/Her3 Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ErbB3/Her3 in ELISAs. Does not cross-react with recombinant EGF R.
  • Source
    Monoclonal Mouse IgG1 Clone # 66223
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
    Ser20-Thr643
    Accession # P21860
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    MCF‑7 human breast cancer cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human ErbB3/Her3 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human ErbB3/Her3 Antibody (Catalog # MAB3481)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human ErbB3/Her3 Biotinylated Antibody (Catalog # BAM348)
  • ELISA Standard
     
    Recombinant Human ErbB3/Her3 Fc Chimera Protein, CF (Catalog # 348-RB)
  • Neutralization
    Measured by its ability to neutralize NRG1‑ beta 1/HRG1‑ beta 1-induced proliferation in the MCF‑7 human breast cancer cell line. The Neutralization Dose (ND50) is typically 0.0075-0.03 µg/mL in the presence of 10 ng/mL Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by NRG1‑ beta 1/HRG1‑ beta 1 and Neutralization by Human ErbB3/Her3 Antibody. Recombinant Human NRG1‑1 beta 1/HRG1‑1 beta 1 (Catalog # 377-HB) stimulates proliferation in the MCF‑17 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1‑1 beta 1/HRG1‑1 beta 1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human ErbB3/Her3 Monoclonal Antibody (Catalog # MAB3481). The ND50 is typically 0.0075-0.03 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ErbB3/Her3

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.

  • References:
    1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
    2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
    3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
    4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
    5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
    6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
    7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
    8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
    9. Schlessinger, J. (2000) Cell 103:211.
    10. Daly, R.J. (1999) Growth Factors 16:255.
  • Long Name:
    Receptor Tyrosine Protein Kinase ErbB3
  • Entrez Gene IDs:
    2065 (Human); 13867 (Mouse)
  • Alternate Names:
    c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Neuregulin 1 allosterically enhances the anti-tumor effects of the non-competing anti-HER3 antibody 9F7-F11 by increasing its binding to HER3
    Authors: C Le Clorenn, H Bazin, O Dubreuil, C Larbouret, C Ogier, Y Lazrek, V Garambois, MA Poul, P Mondon, JM Barret, G Mathis, JF Prost, A Pèlegrin, T Chardès
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  2. A novel antibody engineering strategy for making monovalent bispecific heterodimeric IgG antibodies by electrostatic steering mechanism.
    Authors: Liu Z, Leng E, Gunasekaran K, Pentony M, Shen M, Howard M, Stoops J, Manchulenko K, Razinkov V, Liu H, Fanslow W, Hu Z, Sun N, Hasegawa H, Clark R, Foltz I, Yan W
    J Biol Chem, 2015;290(12):7535-62.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISA Capture
  3. Neuregulin-1-mediated ErbB2-ErbB3 signalling protects human trophoblasts against apoptosis to preserve differentiation
    Authors: V Fock, K Plessl, P Draxler, GR Otti, C Fiala, M Knöfler, J Pollheimer
    J. Cell. Sci, 2015;128(23):4306-16.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Growth response of human colorectal tumour cell lines to treatment with afatinib (BIBW2992), an irreversible erbB family blocker, and its association with expression of HER family members.
    Authors: Khelwatty SA, Essapen S, Seddon AM, Modjtahedi H
    Int. J. Oncol., 2011;39(2):483-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
    Authors: Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA
    Assay Drug Dev Technol, 2010;8(1):27-36.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  6. A 45-kDa ErbB3 secreted by prostate cancer cells promotes bone formation.
    Authors: Lin SH, Cheng CJ, Lee YC, Ye X, Tsai WW, Kim J, Pasqualini R, Arap W, Navone NM, Tu SM, Hu M, Yu-Lee LY, Logothetis CJ
    Oncogene, 2008;27(39):5195-203.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  7. Soluble ErbB3 levels in bone marrow and plasma of men with prostate cancer.
    Authors: Lin SH, Lee YC, Choueiri MB, Wen S, Mathew P, Ye X, Do KA, Navone NM, Kim J, Tu SM, Yu-Lee LY, Logothetis CJ
    Clin. Cancer Res., 2008;14(12):3729-36.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

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Mouse IgG1 Isotype Control

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Secondary Antibodies
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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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