Human ErbB3/Her3 Quantikine ELISA Kit

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DERB30
Human ErbB3/Her3 ELISA Standard Curve
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Human ErbB3/Her3 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (5 ug), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL), Human Milk (10 uL)
Sensitivity
15.2 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human ErbB3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human ErbB3/Her3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ErbB3 in cell culture supernates, cell lysates, serum, plasma, saliva, urine and human milk. It contains NS0-expressed recombinant human ErbB3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ErbB3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ErbB3.

Precision

Intra-Assay Precision (Precision within an assay) <div>Three samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) <div>Three samples of known concentration were tested in twenty separate assays to assess&nbsp;inter-assay precision. Assays were performed by at least three technicians.</div>

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 396 1176 2446 437 1317 2646
Standard Deviation 4.56 11.2 36.9 29.4 66.1 119
CV% 1.2 1 1.5 6.7 5 4.5

Recovery

The recovery of human ErbB3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 92-100
Cell Lysis Buffer (n=2) 98 96-101
EDTA Plasma (n=4) 97 93-103
Heparin Plasma (n=4) 96 91-102
Human Milk (n=4) 96 91-103
Saliva (n=4) 100 94-106
Serum (n=4) 100 93-109
Urine (n=4) 97 94-102

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ErbB3 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human ErbB3/Her3 Linearity

Data Examples

Human ErbB3/Her3 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB3/Her3

ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB3 binds neuregulins NRG1 and NRG2, but lacks a working kinase domain; heterodimerization with another ErbB receptor is required for signaling. ErbB3 is important in development of Schwann cells, neural crest cells, heart valves and the sympathetic nervous system. When expressed with ErbB2 in breast, skin, ovary and gastrointestinal tumors, ErbB3 is a potent mitogen.

Long Name:
Receptor Tyrosine Protein Kinase ErbB3
Entrez Gene IDs:
2065 (Human); 13867 (Mouse)
Alternate Names:
c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

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