Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

Name: Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DFL00B
Price: 680 USD
Availability: InStock

R&D Systems | Catalog # DFL00B

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Product Documents

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

5.1 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)

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Product Summary for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

The Quantikine^® Human Fas Ligand/TNFSF6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fas Ligand in cell culture supernates, cell lysates, serum, and plasma. It contains CHO cell-expressed recombinant human Fas Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fas Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Fas Ligand.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Fas Ligand.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	92.1	240	587	98.6	244	576
Standard Deviation	3.31	7.51	18.2	4.41	8.70	25.7
CV%	3.6	3.1	3.1	4.5	3.6	4.5

Recovery for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

The recovery of human Fas Ligand spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	98	93-103
EDTA Plasma (n=4)	103	94-111
Heparin Plasma (n=4)	96	92-101
Serum (n=4)	101	96-103

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Fas Ligand were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

Human Fas Ligand/TNFSF6 Standard Curve

Human Fas Ligand/TNFSF6 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fas Ligand/TNFSF6

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites. Tumor cells can evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes. In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.

Alternate Names

CD178, CD95L, FASLG, TNFSF6

Entrez Gene IDs

356 (Human); 14103 (Mouse); 25385 (Rat)

Gene Symbol

FASLG

Additional Fas Ligand/TNFSF6 Products

Product Documents for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.