Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Fc gamma RIII (CD16) PE‑conjugated Monoclonal Antibody (Catalog # FAB2546P) and Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fc gamma RIII (CD16)
Fc gamma RIIIA and B are low affinity receptors for polyvalent
immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor
expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB
is a non-activating GPI-linked decoy receptor expressed on neutrophils
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