Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 245536
View all Fc gamma RIII (CD16) Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16)
Thr20-Gln208
Accession # O75015

Specificity

Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Fc gamma RIII (CD16) PE‑conjugated Antibody

Detection of Fc gamma RIII (CD16) antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Fc gamma RIII (CD16) PE-conjugated Monoclonal Antibody (Catalog # FAB2546P) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Detection of Human Fc gamma RIII (CD16) by Flow Cytometry

Detection of Human Fc gamma RIII (CD16) by Flow Cytometry

Analysis of monocyte subsets. A–B. Gated monocytes in a forward vs. side scatter dot-plot analysis of peripheral blood mononuclear cells. Monocytes were then gated according to their surface expression of CD14 and CD16. Flow cytometric analysis of phagocytosis revealed that CD14++CD16+ monocytes (gate III) engulfed polystyrene beads more effectively than the other subsets (gate I and II). CI–III. The mean fluorescence intensities represent the amount of incorporated fluorescent latex particles phagocytosed by 3×105 cells. D. Increase in the percentage of CD14+CD16+ monocytes after 4 h and 8 h of treatment with 31.25 µg/ml glatiramer acetate (GA) in MACS isolated monocytes. Data are expressed as mean percentages ± SEM of three independent experiments. Significant effects vs. controls are indicated by asterisks (*p<0.05, **p<0.01, and ***p<0.001 using Bonferroni's Multiple Comparison Test) as determined by one-way ANOVA. E. Slight but not significant increase of CD16 expression after GA treatment. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23284793), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Fc gamma RIII (CD16) by Flow Cytometry

Detection of Human Fc gamma RIII (CD16) by Flow Cytometry

Analysis of monocyte subsets. A–B. Gated monocytes in a forward vs. side scatter dot-plot analysis of peripheral blood mononuclear cells. Monocytes were then gated according to their surface expression of CD14 and CD16. Flow cytometric analysis of phagocytosis revealed that CD14++CD16+ monocytes (gate III) engulfed polystyrene beads more effectively than the other subsets (gate I and II). CI–III. The mean fluorescence intensities represent the amount of incorporated fluorescent latex particles phagocytosed by 3×105 cells. D. Increase in the percentage of CD14+CD16+ monocytes after 4 h and 8 h of treatment with 31.25 µg/ml glatiramer acetate (GA) in MACS isolated monocytes. Data are expressed as mean percentages ± SEM of three independent experiments. Significant effects vs. controls are indicated by asterisks (*p<0.05, **p<0.01, and ***p<0.001 using Bonferroni's Multiple Comparison Test) as determined by one-way ANOVA. E. Slight but not significant increase of CD16 expression after GA treatment. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23284793), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Fc gamma RIII (CD16) PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 1 review rated 5 using FAB2546P in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RIII (CD16)

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Long Name

Fc gamma Receptor III

Alternate Names

FcgRIII

Entrez Gene IDs

14131 (Mouse); 102140945 (Cynomolgus Monkey)

Gene Symbol

FCGR3

UniProt

O75015

Additional Fc gamma RIII (CD16) Products

Product Documents for Human Fc gamma RIII (CD16) PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Fc gamma RIII (CD16) PE‑conjugated Antibody

For research use only

Citations for Human Fc gamma RIII (CD16) PE‑conjugated Antibody

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  • Human Fc gamma RIII (CD16) Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human PBMC
    Species: Human
    Verified Customer | Posted 03/01/2019
    Human PBMCs were stained with FcgRIII PE antibody and plotted vs. FSC
    PBMCs were stained w ith anti-hFcgRIII A/B PE.
    Human Fc gamma RIII (CD16) PE‑conjugated Antibody FAB2546P

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