Human Fc gamma RIII (CD16) Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 245536
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16)
    Thr20-Gln208
    Accession # O75015
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Fc gamma RIII (CD16) Biotinylated Monoclonal Antibody (Catalog # FAB2546B) or (B) Mouse IgG2A Biotinylated Isotype Control (Catalog # IC003B) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fc gamma RIII (CD16)
CD16 (also known as FcgRIII) is a CD designation that, in human, encompasses two related, but distinct Ig superfamily genes; CD16a and CD16b. Mature human CD16a is a 45-75 kDa type I transmembrane glycoprotein that is 238 amino acids (aa) in length. It contains a 192 aa extracellular region that possesses two Ig-like domains plus glycosylation that is critical to its function, and ends with a short 25 aa cytoplasmic domain. On the cell surface, CD16a complexes with FceRI and CD3 zeta. CD16a shows a restricted expression pattern, being found on immature thymocytes, placental trophoblast cells, CD56dim and cytokine-activated CD56+ NK cells, slan (sulfated NAcLac)-expressing dendritic cells, and CX3CR1+ CD14+/- "patrolling" monocytes. Mature CD16b, by contrast, is slightly smaller that CD16a, but still shows the same 50-70 kSDa electrophoretic pattern in SDS-PAGE. It is a GPI-linked glycoprotein that is 184 aa in length. On the cell surface, CD16b is monomeric. The standard allele (= NA2) is encoded by the SwissProt Accession # used for this product (see above). The two variant alleles are NA1, which shows four aa substitutions, and SH, which contains only one aa substitution relative to the NA2 sequence. CD16b expression appears to be limited to neutrophils. CD16b is known to be solubilized, and the soluble form is believed to arise from the action of ADAM-17 rather than a phospholipase. Over aa 20-208, human CD16a and CD16b share 97% aa sequence identity. Over the same aa range, human CD16b and mouse CD16 share only 47% aa sequence identity.
  • Long Name:
    Fc gamma Receptor III
  • Entrez Gene IDs:
    14131 (Mouse); 102140945 (Cynomolgus Monkey)
  • Alternate Names:
    Fc gamma RIII; FcgRIII
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Biotinylated Isotype Control

Ctrl IC003B
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 4
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Streptavidin-Phycoerythrin

Flow F0040 5
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Streptavidin-Alkaline Phosphatase

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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 3
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Avidin-HRP

WB A-115 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
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Streptavidin-HRP (set of 5 vials enough for 75 plates total)

ELISA DY998 37
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EvenCoat Streptavidin Coated Plates, 15 Pack

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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