Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Fc gamma RIII (CD16) Biotinylated Monoclonal Antibody (Catalog # FAB2546B) or (B) Mouse IgG2A Biotinylated Isotype Control (Catalog # IC003B) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fc gamma RIII (CD16)
CD16 (also known as FcgRIII)
is a CD designation that, in human, encompasses two related, but distinct Ig
superfamily genes; CD16a and CD16b.
Mature human CD16a is a 45-75 kDa type I transmembrane glycoprotein that
is 238 amino acids (aa) in length. It
contains a 192 aa extracellular region that possesses two Ig-like domains plus
glycosylation that is critical to its function, and ends with a short 25 aa
cytoplasmic domain. On the cell surface,
CD16a complexes with FceRI and CD3 zeta.
CD16a shows a restricted expression pattern, being found on immature
thymocytes, placental trophoblast cells, CD56dim and
cytokine-activated CD56+ NK cells, slan (sulfated NAcLac)-expressing dendritic cells, and CX3CR1+
CD14+/- "patrolling" monocytes.
Mature CD16b, by contrast, is slightly smaller that CD16a, but still
shows the same 50-70 kSDa electrophoretic pattern in SDS-PAGE. It is a GPI-linked glycoprotein that is 184
aa in length. On the cell surface, CD16b
is monomeric. The standard allele (=
NA2) is encoded by the SwissProt Accession # used for this product (see
above). The two variant alleles are NA1,
which shows four aa substitutions, and SH, which contains only one aa
substitution relative to the NA2 sequence. CD16b expression appears to be limited
to neutrophils. CD16b is known to be
solubilized, and the soluble form is believed to arise from the action of
ADAM-17 rather than a phospholipase.
Over aa 20-208, human CD16a and CD16b share 97% aa sequence
identity. Over the same aa range, human
CD16b and mouse CD16 share only 47% aa sequence identity.
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