Human Fc gamma RIII (CD16) Antibody
Human Fc gamma RIII (CD16) Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Fc gamma RIIIA/B (CD16a/b) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Fc gamma RIII A/B (CD16a/b) Monoclonal Antibody (Catalog # MAB2546) or (B) Mouse IgG2A isotype control antibody (MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fc gamma RIII (CD16)
Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.
Product Datasheets
Citations for Human Fc gamma RIII (CD16) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-?
Authors: KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten
Eur. J. Immunol, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
FcgammaRIIIa-Syk Co-signal Modulates CD4+ T-cell Response and Up-regulates Toll-like Receptor (TLR) Expression.
Authors: Chauhan A, Moore T, Bi Y, Chen C
J Biol Chem, 2016;291(3):1368-86.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Evidence for chemokine synergy during neutrophil migration in ARDS
Thorax, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Induced expression of FcgammaRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-gammahigh subset.
Authors: Chauhan A, Chen C, Moore T, DiPaolo R
J Biol Chem, 2015;290(8):5127-40.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients.
Authors: Mou, Xiao, Zhou, Yuepeng, Jiang, Peng, Zhou, Tong, Jiang, Qian, Xu, Chengche, Liu, Hongli, Zheng, Tingting, Yuan, Guoyue, Zhang, Yanyun, Chen, Deyu, Mao, Chaoming
Sci Rep, 2014;4(0):6138.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Immune complexes and late complement proteins trigger activation of Syk tyrosine kinase in human CD4(+) T cells.
Authors: Chauhan AK, Moore TL
Clin. Exp. Immunol., 2012;167(2):235-45.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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