Human Fc gamma RIII (CD16) Antibody

Catalog # Availability Size / Price Qty
MAB2546-SP
MAB2546-100
MAB2546-500
Detection of Fc gamma RIIIA/B (CD16a/b) in Human PBMCs by Flow Cytometry.
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Product Details
Citations (6)
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Human Fc gamma RIII (CD16) Antibody Summary

Species Reactivity
Human
Source
Monoclonal Mouse IgG2A Clone # 245536
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Raised against Fc gamma RIII (CD16).
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human whole blood granulocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Fc gamma RIIIA/B (CD16a/b) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Fc gamma RIII A/B (CD16a/b) Monoclonal Antibody (Catalog # MAB2546) or (B) Mouse IgG2A isotype control antibody (MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Functional Detection of Human Fc gamma RIII (CD16) by Functional View Larger

Detection of Human Fc gamma RIII (CD16) by Functional Anti-ULBP3 monoclonal antibody enhances the activity of NK cells from cancer patients via ADCC.ULBP3 expression and the percentage of infiltrating NK cells in tumor tissues from 10 patients (4 colorectal cancer, 3 lung cancer, and 3 gastric cancer) were determined by FCM. A representative graph is shown in (A). The relationship between ULBP3 expression and infiltrating NK cells in tumor tissues is shown in (B). Normal NK cells were co-cultured with CD133−SW620 (C) or 7721 (D) cells at the indicated E:T ratios, and cytotoxicity was measured by LDH release after 6 h of co-culture, with or without the addition of isotype IgG, anti-ULBP3 (B2-F1-F1), and/or anti-CD16 (5 μg/ml). K562 (E) or A549 (F) cells were co-cultured with normal NK cells, and cytotoxicity was measured by LDH release after 6 h of co-culture at the indicated E:T ratios, with or without the addition of ULBP3-Fc (1 ng/ml) and/or B2-F1-F1 (5 μg/ml). The data are representative of results obtained from at least 3 independent experiments. The results are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep06138), licensed under a CC-BY license. Not internally tested by R&D Systems.

Functional Detection of Human Fc gamma RIII (CD16) by Functional View Larger

Detection of Human Fc gamma RIII (CD16) by Functional Anti-ULBP3 monoclonal antibody enhances the activity of NK cells from cancer patients via ADCC.ULBP3 expression and the percentage of infiltrating NK cells in tumor tissues from 10 patients (4 colorectal cancer, 3 lung cancer, and 3 gastric cancer) were determined by FCM. A representative graph is shown in (A). The relationship between ULBP3 expression and infiltrating NK cells in tumor tissues is shown in (B). Normal NK cells were co-cultured with CD133−SW620 (C) or 7721 (D) cells at the indicated E:T ratios, and cytotoxicity was measured by LDH release after 6 h of co-culture, with or without the addition of isotype IgG, anti-ULBP3 (B2-F1-F1), and/or anti-CD16 (5 μg/ml). K562 (E) or A549 (F) cells were co-cultured with normal NK cells, and cytotoxicity was measured by LDH release after 6 h of co-culture at the indicated E:T ratios, with or without the addition of ULBP3-Fc (1 ng/ml) and/or B2-F1-F1 (5 μg/ml). The data are representative of results obtained from at least 3 independent experiments. The results are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep06138), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIII (CD16)

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Long Name
Fc gamma Receptor III
Entrez Gene IDs
14131 (Mouse); 102140945 (Cynomolgus Monkey)
Alternate Names
CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

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Citations for Human Fc gamma RIII (CD16) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-?
    Authors: KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten
    Eur. J. Immunol, 2017-03-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Evidence for chemokine synergy during neutrophil migration in ARDS
    Thorax, 2016-08-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. FcgammaRIIIa-Syk Co-signal Modulates CD4+ T-cell Response and Up-regulates Toll-like Receptor (TLR) Expression.
    Authors: Chauhan A, Moore T, Bi Y, Chen C
    J Biol Chem, 2015-11-18;291(3):1368-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Induced expression of FcgammaRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-gammahigh subset.
    Authors: Chauhan A, Chen C, Moore T, DiPaolo R
    J Biol Chem, 2015-01-02;290(8):5127-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients.
    Authors: Mou, Xiao, Zhou, Yuepeng, Jiang, Peng, Zhou, Tong, Jiang, Qian, Xu, Chengche, Liu, Hongli, Zheng, Tingting, Yuan, Guoyue, Zhang, Yanyun, Chen, Deyu, Mao, Chaoming
    Sci Rep, 2014-08-20;4(0):6138.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  6. Immune complexes and late complement proteins trigger activation of Syk tyrosine kinase in human CD4(+) T cells.
    Authors: Chauhan AK, Moore TL
    Clin. Exp. Immunol., 2012-02-01;167(2):235-45.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation

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