Human FCRN Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC8639R-100UG
Detection  of FCRN in U937 Human Cell Line by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human FCRN Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FCRN in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 937508
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human FCRN
Ala24-Ser297
Accession # P55899
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of FCRN in U937 Human Cell Line by Flow Cytometry. View Larger

Detection of FCRN in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human FCRN Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8639R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FCRN

The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells (1, 2). Mature human FCRN consists of a 274 amino acid (aa) extracellular domain (ECD) with two N-terminal alpha domains, one alpha 3/immunoglobulin-like domain, a 23 aa transmembrane segment, and a 44 aa cytoplasmic domain (3). Within the ECD, human FCRN shares 68% aa sequence identity with mouse and rat FCRN. Mouse FCRN binds with high affinity to IgG from mouse, human, rat, rabbit, guinea pig, bovine, and sheep, while human FCRN binds IgG with significantly lower affinity and is much more restricted in terms of species recognition (4). It does not bind the structurally related chicken IgY (5). FCRN additionally binds to albumin, and both it and IgG are bound at pH 5.0 but not at pH 8.0 (3, 6). FCRN associates noncovalently with beta 2-Microglobulin, and this interaction is important for the intracellular trafficking of FCRN (7-10). FCRN cycles between the plasma membrane and acidified intracellular compartments of endothelial cells and epithelial cells (5, 8). It binds endocytosed IgG and albumin in the low pH vesicles and transports them to the plasma membrane for extracellular release at higher pH. This protects IgG and albumin from lysosomal degradation and helps maintain the circulating levels of both proteins (5, 6). This mechanism is involved in the bidirectional transport of IgG across epithelial and endothelial barriers including neonatal IgG absorption in the intestine and fetal uptake of maternal antibodies through the placenta (5, 8, 11, 12). In the kidney, FCRN recycles albumin to the serum but removes IgG from the glomular basement membrane and promotes its excretion into the urine (13, 14). FCRN is also expressed in neutrophils and myeloid antigen presenting cells (7, 15, 16). It can enhance IgG-mediated phagocytosis and antigen presentation by these cells, but it promotes the degradation of opsonizing IgG rather than returning it to the circulation (15, 16).

References
  1. Baker, K. et al. (2009) Semin. Immunopathol. 31:223.
  2. Baker, K. et al. (2014) Front. Immunol. 5:408.
  3. Story, C.M. et al. (1994) J. Exp. Med. 180:2377.
  4. Ober, R.J. et al. (2001) Int. Immunol. 13:1551.
  5. Dickinson, B.L. et al. (1999) J. Clin. Invest. 104:903.
  6. Chaudhury, C. et al. (2003) J. Exp. Med. 197:315.
  7. Simister, N.E. and K.E. Mostov (1989) Nature 337:184.
  8. Kobayashi, N. et al. (2002) Am. J. Physiol. Renal Physiol. 282:F358.
  9. Praetor, A. and W. Hunziker (2002) J. Cell Sci. 115:2389.
  10. Zhu, X. et al. (2001) J. Immunol. 166:3266.
  11. Spiekermann, G.M. et al. (2002) J. Exp. Med. 196:303.
  12. Firan, M. et al. (2001) Int. Immunol. 13:993.
  13. Sarav, M. et al. (2009) J. Am. Soc. Nephrol. 20:1941.
  14. Akilesh, S. et al. (2008) Proc. Natl. Acad. Sci. 105:967.
  15. Vidarsson, G. et al. (2006) Blood 108:3573.
  16. Qiao, S.-W. et al. (2008) Proc. Natl. Acad. Sci. 105:9337.
Long Name
Neonatal Fc Receptor
Entrez Gene IDs
2217 (Human); 14132 (Mouse); 29558 (Rat); 102128913 (Cynomolgus Monkey)
Alternate Names
alpha-chain; Fc fragment of IgG, receptor, transporter, alpha; FCGRT; FcRn alpha chain; FCRN; FCRNimmunoglobulin receptor, intestinal, heavy chain; IgG Fc fragment receptor transporter alpha chain; IgG receptor FcRn large subunit p51; major histocompatibility complex class I-like Fc receptor; Neonatal Fc receptor; neonatal Fc-receptor for Ig

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human FCRN Alexa Fluor® 647-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human FCRN Alexa Fluor® 647-conjugated Antibody and earn rewards!

Have you used Human FCRN Alexa Fluor® 647-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review