Human FGF-21 Quantikine ELISA Kit

(8 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    8.69 pg/mL
  • Assay Range
    31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human FGF-21
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC24, Quantikine Immunoassay Control Group 9 - Please Inquire
Product Summary
The Quantikine Human FGF-21 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF-21 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF-21 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FGF-21 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FGF-21.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean20467213242457421411
Standard Deviation819.846.226.647.273.9
CV%3.92.93.510.96.45.2

Recovery

The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 93-108
EDTA Plasma (n=4) 103 92-112
Heparin Plasma (n=4) 98 83-111
Serum (n=4) 105 87-119
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FGF-21 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF-21
FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.
    • Long Name
      Fibroblast Growth Factor 21
    • Entrez Gene IDs
      26291 (Human); 56636 (Mouse);
    • Alternate Names
      FGF21; FGF-21; fibroblast growth factor 21;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    8 Citations: Showing 1 - 8
    Filter your results:

    Species
    Sample Type
    1. The Relationship between Mitochondrial Respiratory Chain Activities in Muscle and Metabolites in Plasma and Urine: A Retrospective Study
      Authors: C Alban, E Fatale, A Joulani, P Ilin, A Saada
      J Clin Med, 2017;6(3):.
      Species: Human
      Sample Type: Plasma
    2. Circulating FGF21 in humans is potently induced by short term overfeeding of carbohydrates
      Authors: AM Lundsgaard, AM Fritzen, KA Sj?berg, LS Myrmel, L Madsen, JF Wojtaszews, EA Richter, B Kiens
      Mol Metab, 2017;6(1):22-29.
      Species: Human
      Sample Type: Plasma
    3. Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases
      Authors: T Matsuhashi, T Sato, SI Kanno, T Suzuki, A Matsuo, Y Oba, M Kikusato, E Ogasawara, T Kudo, K Suzuki, O Ohara, H Shimbo, F Nanto, H Yamaguchi, D Saigusa, Y Mukaiyama, A Watabe, K Kikuchi, H Shima, E Mishima, Y Akiyama, Y Oikawa, HO Hsin-Jung, Y Akiyama, C Suzuki, M Uematsu, M Ogata, N Kumagai, M Toyomizu, A Hozawa, N Mano, Y Owada, S Aiba, T Yanagisawa, Y Tomioka, S Kure, S Ito, K Nakada, KI Hayashi, H Osaka, T Abe
      EBioMedicine, 2017;0(0):.
      Species: Human
      Sample Type: Serum
    4. Raised FGF-21 and Triglycerides Accompany Increased Energy Intake Driven by Protein Leverage in Lean, Healthy Individuals: A Randomised Trial
      PLoS ONE, 2016;11(8):e0161003.
      Species: Human
      Sample Type: Plasma
    5. CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis
      Authors: Jong-Gil Park
      Sci Rep, 2016;6(0):27938.
      Species: Mouse
      Sample Type: Plasma
    6. Lack of Day/Night variation in fibroblast growth factor 21 levels in young healthy men.
      Authors: Foo J, Aronis K, Chamberland J, Mantzoros C
      Int J Obes (Lond), 2015;39(6):945-8.
      Species: Human
      Sample Type: Serum
    7. Effect of endurance training on skeletal muscle myokine expression in obese men: identification of apelin as a novel myokine.
      Authors: Besse-Patin, A, Montastier, E, Vinel, C, Castan-Laurell, I, Louche, K, Dray, C, Daviaud, D, Mir, L, Marques, M-A, Thalamas, C, Valet, P, Langin, D, Moro, C, Viguerie, N
      Int J Obes (Lond), 2014;38(5):707-13.
      Species: Human
      Sample Type: Plasma
    8. Serum concentrations of fibroblast growth factors 19 and 21 in women with gestational diabetes mellitus: association with insulin resistance, adiponectin, and polycystic ovary syndrome history.
      Authors: Wang D, Zhu W, Li J, An C, Wang Z
      PLoS ONE, 2013;8(11):e81190.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Group 9

    Ctrl QC24
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Average Rating: 4 (Based on 1 review)

    Have you used Human FGF-21 Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Read what people are saying who have used Human FGF-21 Quantikine ELISA Kit.

    Filter by Ratings
    Very Good
    (1)
    All Ratings
    Filter by Applications
    All Applications
    Filter by Species
    All Species

    Images Ratings Applications Species Reviewed By Date Details
      Very Good
      Anonymous 10/23/2017
    Show

    Summary

    Sample TestedSerum

    Order Details

    Contact Information

    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed

    Feedback

    Customer Information