Human FGF-21 Quantikine ELISA Kit

  (11 citations)
(1 Review)
    
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Product Details
Assay Procedure
Citations (11)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    8.69 pg/mL
  • Assay Range
    31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human FGF-21
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC24 , Quantikine Immunoassay Control Group 9 - Please Inquire
Product Summary
The Quantikine Human FGF-21 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF-21 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF-21 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FGF-21 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FGF-21.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 204 672 1324 245 742 1411
Standard Deviation 8 19.8 46.2 26.6 47.2 73.9
CV% 3.9 2.9 3.5 10.9 6.4 5.2

Recovery

The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 93-108
EDTA Plasma (n=4) 103 92-112
Heparin Plasma (n=4) 98 83-111
Serum (n=4) 105 87-119
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 FGF-21 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF-21
FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.
  • Long Name:
    Fibroblast Growth Factor 21
  • Entrez Gene IDs:
    26291 (Human); 56636 (Mouse)
  • Alternate Names:
    FGF21; FGF-21; fibroblast growth factor 21
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Sample Type
  1. FGF21, a liver hormone that inhibits alcohol intake in mice, increases in human circulation after acute alcohol ingestion and sustained binge drinking at Oktoberfest
    Authors: S Søberg, ES Andersen, NB Dalgaard, I Jarlhelt, NL Hansen, N Hoffmann, T Vilsbøll, A Chenchar, M Jensen, TJ Grevengoed, SAJ Trammell, FK Knop, MP Gillum
    Mol Metab, 2018;0(0):.
    Species: Human
    Sample Type: Plasma
  2. Epigenetic modulation of Fgf21 in the perinatal mouse liver ameliorates diet-induced obesity in adulthood
    Authors: X Yuan, K Tsujimoto, K Hashimoto, K Kawahori, N Hanzawa, M Hamaguchi, T Seki, M Nawa, T Ehara, Y Kitamura, I Hatada, M Konishi, N Itoh, Y Nakagawa, H Shimano, T Takai-Igar, Y Kamei, Y Ogawa
    Nat Commun, 2018;9(1):636.
    Species: Mouse
    Sample Type: Serum
  3. The Relationship between Mitochondrial Respiratory Chain Activities in Muscle and Metabolites in Plasma and Urine: A Retrospective Study
    Authors: C Alban, E Fatale, A Joulani, P Ilin, A Saada
    J Clin Med, 2017;6(3):.
    Species: Human
    Sample Type: Plasma
  4. Circulating FGF21 in humans is potently induced by short term overfeeding of carbohydrates
    Authors: AM Lundsgaard, AM Fritzen, KA Sj?berg, LS Myrmel, L Madsen, JF Wojtaszews, EA Richter, B Kiens
    Mol Metab, 2017;6(1):22-29.
    Species: Human
    Sample Type: Plasma
  5. Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases
    Authors: T Matsuhashi, T Sato, SI Kanno, T Suzuki, A Matsuo, Y Oba, M Kikusato, E Ogasawara, T Kudo, K Suzuki, O Ohara, H Shimbo, F Nanto, H Yamaguchi, D Saigusa, Y Mukaiyama, A Watabe, K Kikuchi, H Shima, E Mishima, Y Akiyama, Y Oikawa, HO Hsin-Jung, Y Akiyama, C Suzuki, M Uematsu, M Ogata, N Kumagai, M Toyomizu, A Hozawa, N Mano, Y Owada, S Aiba, T Yanagisawa, Y Tomioka, S Kure, S Ito, K Nakada, KI Hayashi, H Osaka, T Abe
    EBioMedicine, 2017;0(0):.
    Species: Human
    Sample Type: Serum
  6. Fibroblast growth factor 21 (FGF21) is robustly induced by ethanol and has a protective role in ethanol associated liver injury
    Authors: BN Desai, G Singhal, M Watanabe, D Stevanovic, T Lundasen, FM Fisher, ML Mather, HG Vardeh, N Douris, AC Adams, IA Nasser, GA FitzGerald, JS Flier, C Skarke, E Maratos-Fl
    Mol Metab, 2017;6(11):1395-1406.
    Species: Human
    Sample Type: Serum
  7. Raised FGF-21 and Triglycerides Accompany Increased Energy Intake Driven by Protein Leverage in Lean, Healthy Individuals: A Randomised Trial
    PLoS ONE, 2016;11(8):e0161003.
    Species: Human
    Sample Type: Plasma
  8. CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis
    Authors: Jong-Gil Park
    Sci Rep, 2016;6(0):27938.
    Species: Mouse
    Sample Type: Plasma
  9. Lack of Day/Night variation in fibroblast growth factor 21 levels in young healthy men.
    Authors: Foo J, Aronis K, Chamberland J, Mantzoros C
    Int J Obes (Lond), 2015;39(6):945-8.
    Species: Human
    Sample Type: Serum
  10. Effect of endurance training on skeletal muscle myokine expression in obese men: identification of apelin as a novel myokine.
    Authors: Besse-Patin, A, Montastier, E, Vinel, C, Castan-Laurell, I, Louche, K, Dray, C, Daviaud, D, Mir, L, Marques, M-A, Thalamas, C, Valet, P, Langin, D, Moro, C, Viguerie, N
    Int J Obes (Lond), 2014;38(5):707-13.
    Species: Human
    Sample Type: Plasma
  11. Serum concentrations of fibroblast growth factors 19 and 21 in women with gestational diabetes mellitus: association with insulin resistance, adiponectin, and polycystic ovary syndrome history.
    Authors: Wang D, Zhu W, Li J, An C, Wang Z
    PLoS ONE, 2013;8(11):e81190.
    Species: Human
    Sample Type: Serum
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Average Rating: 4 (Based on 1 review)

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