Human FGFR3 Alexa Fluor® 647-conjugated Antibody

Clone 136334 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB766R-100UG
Detection of FGF R3 in K562 Human Cell Line by Flow Cytometry.
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Human FGFR3 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects the IIIb and IIIc isoforms of human FGF R3 in direct ELISAs and Western blots. Does not cross-react with any isoforms of recombinant mouse (rm) FGF R3, rmFGF R2, recombinant human (rh) FGF R1, rhFGF R2, or rhFGF R4.
Source
Monoclonal Mouse IgG1 Clone # 136334
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0- and Sf21-derived recombinant human FGF R3 alpha (IIIb)
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of FGF R3 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human FGF R3 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB766R, filled histogram) or isotype control antibody (IC002R, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitution default not known
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FGFR3

Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co‑factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF -1, -2, -4, -8b, -8e, -8f, -9, and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Long Name
Fibroblast Growth Factor Receptor 3
Entrez Gene IDs
2261 (Human); 14184 (Mouse)
Alternate Names
CD333; CEK; EC 2.7.10; FGF R3; FGFR3; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism

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