Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 136334
View all FGFR3 Primary Antibodies »

Product Specifications

Immunogen

Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)

Specificity

Detects the IIIb and IIIc isoforms of human FGF R3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FGFR3 Antibody

Detection of FGFR3 in HepG2 cells by Flow Cytometry

HepG2 cells were stained with Mouse Anti-Human FGFR3 Monoclonal Antibody (Catalog # MAB766, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse FGFR3 by Western Blot

Detection of Mouse FGFR3 by Western Blot

TAB cells modulate melanoma cells to express FGFR-3 and its ligand FGF-2 for tumor stroma-tumor cell cross-talk. a WM3749 co-cultured (72 h) with TAB cells (red bar) show increased FGFR-3 mRNA expression when compared with tumors only (open bar) or co-cultured with NB cells (blue bar). b Lysates obtained from pools of melanomas co-cultured (72–120 h) with NB- or TAB cells were probed in western blot with anti-FGFR-3 antibody (left panel), results expressed as relative intensity after beta -actin normalization (right panel). c Melanoma cells co-cultured with TAB cells (72 h) show increased phospho-FGFR-3 expression (right panel; immunofluorescence assays) when compared with melanoma cells alone (left panel) or melanoma cells co-cultured with NB cells (middle panel), scale bars: 40 μm, images captured by Nikon inverted microscope. d Melanoma cells co-cultured with TAB cells (red bar) show increased FGF-2 mRNA expression when compared with melanoma cells alone (open bar) or melanoma cells co-cultured with NB cells (blue bar). e 451Lu and WM989treated with IGF-1 (25 ng/ml/daily for 5 days; red bar) show increased FGFR-3 expression when compared with untreated controls (blue bar), flow cytometry results expressed as net % expression of control antibody. IGF-1 treated melanoma cells (red bars) show higher expression of FGFR-3 compared with untreated cells (blue bars). Bar represents mean + SD of replicate samples. f NB cells treated with FGF-2 (10 ng/ml/daily for 4 days; red bar) show high IGF-1 mRNA expression relative to untreated NB cells (blue bar). g 451Lu and WM989 co-cultured (72 h) with TAB cells in the presence of an anti-IGF-1 neutralizing antibody (10 μg/ml) show decreased FGFR-3 mRNA expression in tumor cells (blue bar) when compared with controls (red bar). h TAB cells co-cultured (72 h) with 451Lu and WM989 in the presence of an anti-FGF-2 neutralizing antibody (1 μg/ml) show decreased IGF-1 mRNA expression in B cells (blue bar) when compared with controls (red bar).Experiments in a, d and f–h were performed using qPCR. In Figures a, d–h, bars represent mean + SE of duplicate samples and are representative of at least two independent experiments. i Summary of cross-talk between melanoma and B cells: FGF-2 is constitutively expressed by tumor cells, released into the microenvironment to bind FGFR-3 on the B cells, activated B cells express increased levels of pro-inflammatory cytokines. IGF-1 released by TAB cells modulates tumor cells to increase their growth, heterogeneity and therapy resistance Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28928360), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human FGFR3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR)
Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR)

Reviewed Applications

Read 2 reviews rated 4 using MAB766 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR3

Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co‑factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF -1, -2, -4, -8b, -8e, -8f, -9, and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Long Name

Fibroblast Growth Factor Receptor 3

Alternate Names

CD333, CEK, FGF R3, JTK4

Entrez Gene IDs

2261 (Human); 14184 (Mouse)

Gene Symbol

FGFR3

Additional FGFR3 Products

Product Documents for Human FGFR3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FGFR3 Antibody

For research use only

Citations for Human FGFR3 Antibody

Customer Reviews for Human FGFR3 Antibody (2)

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Customer Images

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  • Human FGF R3 Antibody
    Name: Denis Belitskin
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line, T47D human breast cancer cell line, MCF 10A human breast epithelial cell line, MDA-MB-361 and MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 04/20/2019
    Analysis expression of the protein in breast cancer cell lines
    Human FGFR3 Antibody MAB766
  • Name: Natalie Erdmann
    Application: Immunocytochemistry
    Sample Tested: KMS11, KMS18 and RPMI8226 (multiple myeloma) cell lines
    Species: Human
    Verified Customer | Posted 01/28/2016
    IFF/ICC staining of KMS11, KMS18 and RPMI8226 cells with MAB766
    Human FGFR3 Antibody MAB766

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FAQs for Human FGFR3 Antibody

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  • Q: The immunogen for Human FGFR3 Antibody, Catalog # MAB766 is described as "Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)". What does this mean?

    A: To generate Catalog # MAB766, we used a pool of two proteins (NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)) as the immunogen. This antibody detects the IIIb and IIIc isoforms of human FGF R3 in direct ELISAs and Western blots.

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