Human FGFR3 Antibody

Clone 136334 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB766-SP
MAB766-500
MAB766-100

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Detection of Mouse FGFR3 by Western Blot
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Product Details
Citations (4)
FAQs
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Reviews (1)

Human FGFR3 Antibody Summary

Species Reactivity
Human
Specificity
Detects the IIIb and IIIc isoforms of human FGF R3 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 136334
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR)
Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR)
Flow Cytometry
2.5 µg/106 cells
K562 human chronic myelogenous leukemia cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse FGFR3 by Western Blot View Larger

Detection of Mouse FGFR3 by Western Blot TAB cells modulate melanoma cells to express FGFR-3 and its ligand FGF-2 for tumor stroma-tumor cell cross-talk. a WM3749 co-cultured (72 h) with TAB cells (red bar) show increased FGFR-3 mRNA expression when compared with tumors only (open bar) or co-cultured with NB cells (blue bar). b Lysates obtained from pools of melanomas co-cultured (72–120 h) with NB- or TAB cells were probed in western blot with anti-FGFR-3 antibody (left panel), results expressed as relative intensity after beta -actin normalization (right panel). c Melanoma cells co-cultured with TAB cells (72 h) show increased phospho-FGFR-3 expression (right panel; immunofluorescence assays) when compared with melanoma cells alone (left panel) or melanoma cells co-cultured with NB cells (middle panel), scale bars: 40 μm, images captured by Nikon inverted microscope. d Melanoma cells co-cultured with TAB cells (red bar) show increased FGF-2 mRNA expression when compared with melanoma cells alone (open bar) or melanoma cells co-cultured with NB cells (blue bar). e 451Lu and WM989treated with IGF-1 (25 ng/ml/daily for 5 days; red bar) show increased FGFR-3 expression when compared with untreated controls (blue bar), flow cytometry results expressed as net % expression of control antibody. IGF-1 treated melanoma cells (red bars) show higher expression of FGFR-3 compared with untreated cells (blue bars). Bar represents mean + SD of replicate samples. f NB cells treated with FGF-2 (10 ng/ml/daily for 4 days; red bar) show high IGF-1 mRNA expression relative to untreated NB cells (blue bar). g 451Lu and WM989 co-cultured (72 h) with TAB cells in the presence of an anti-IGF-1 neutralizing antibody (10 μg/ml) show decreased FGFR-3 mRNA expression in tumor cells (blue bar) when compared with controls (red bar). h TAB cells co-cultured (72 h) with 451Lu and WM989 in the presence of an anti-FGF-2 neutralizing antibody (1 μg/ml) show decreased IGF-1 mRNA expression in B cells (blue bar) when compared with controls (red bar).Experiments in a, d and f–h were performed using qPCR. In Figures a, d–h, bars represent mean + SE of duplicate samples and are representative of at least two independent experiments. i Summary of cross-talk between melanoma and B cells: FGF-2 is constitutively expressed by tumor cells, released into the microenvironment to bind FGFR-3 on the B cells, activated B cells express increased levels of pro-inflammatory cytokines. IGF-1 released by TAB cells modulates tumor cells to increase their growth, heterogeneity and therapy resistance Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28928360), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR3

Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co‑factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF -1, -2, -4, -8b, -8e, -8f, -9, and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Long Name
Fibroblast Growth Factor Receptor 3
Entrez Gene IDs
2261 (Human); 14184 (Mouse)
Alternate Names
CD333; CEK; EC 2.7.10; FGF R3; FGFR3; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism

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Citations for Human FGFR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells
    Authors: Jakl, V;Popp, T;Haupt, J;Port, M;Roesler, R;Wiese, S;Friemert, B;Rojewski, MT;Schrezenmeier, H;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
    Authors: BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd
    Sci Rep, 2019-03-20;9(1):4902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Tumor-associated B-cells induce tumor heterogeneity and therapy resistance
    Authors: R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner
    Nat Commun, 2017-09-19;8(1):607.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Diagnostic evaluation of t(4;14) in multiple myeloma and evidence for clonal evolution.
    Authors: Stewart AK, Chang H, Trudel S
    Leukemia, 2007-06-14;21(11):2358-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. The immunogen for Human FGFR3 Antibody, Catalog # MAB766 is described as "Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)". What does this mean?

    • To generate Catalog # MAB766, we used a pool of two proteins (NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)) as the immunogen. This antibody detects the IIIb and IIIc isoforms of human FGF R3 in direct ELISAs and Western blots.

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Human FGF R3 Antibody
By Denis Belitskin on 04/20/2019
Application: WB Sample Tested: MCF 10A human breast epithelial cell line,MCF-7 human breast cancer cell line,MDA-MB-231 human breast cancer cell line,MDA-MB-361,T47D human breast cancer cell line Species: Human

Analysis expression of the protein in breast cancer cell lines