Human FGFR3 Antibody

Clone 136334 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB766-SP
MAB766-500
MAB766-100
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human FGFR3 Antibody Summary

Species Reactivity
Human
Specificity
Detects the IIIb and IIIc isoforms of human FGF R3 in direct ELISAs and Western blots. Does not cross-react with any isoforms of recombinant mouse (rm) FGF R3, rmFGF R2, recombinant human (rh) FGF R1, rhFGF R2, or rhFGF R4.
Source
Monoclonal Mouse IgG1 Clone # 136334
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0- and Sf21-derived recombinant human FGF R3 alpha (IIIb)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR)
Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR)
Flow Cytometry
2.5 µg/106 cells
K562 human chronic myelogenous leukemia cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR3

Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co‑factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF -1, -2, -4, -8b, -8e, -8f, -9, and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Long Name
Fibroblast Growth Factor Receptor 3
Entrez Gene IDs
2261 (Human); 14184 (Mouse)
Alternate Names
CD333; CEK; EC 2.7.10; FGF R3; FGFR3; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism

Product Datasheets

Citations for Human FGFR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
    Authors: BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd
    Sci Rep, 2019;9(1):4902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Tumor-associated B-cells induce tumor heterogeneity and therapy resistance
    Authors: R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner
    Nat Commun, 2017;8(1):607.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Diagnostic evaluation of t(4;14) in multiple myeloma and evidence for clonal evolution.
    Authors: Stewart AK, Chang H, Trudel S
    Leukemia, 2007;21(11):2358-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human FGF R3 Antibody
By Anonymous on 04/20/2019
Application: WB Sample Tested: MCF 10A human breast epithelial cell line,MCF-7 human breast cancer cell line,MDA-MB-231 human breast cancer cell line,MDA-MB-361,T47D human breast cancer cell line Species: Human

Analysis expression of the protein in breast cancer cell lines