Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, FACS, Flow Cytometry Control

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 427819
View all Fibroblast Activation Protein alpha/FAP Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
Leu26-Asp760
Accession # Q12884

Specificity

Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPP6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Detection of Fibroblast Activation Protein a/FAP antibody in WI‑38 Human Cell Line antibody by Flow Cytometry.

Detection of Fibroblast Activation Protein alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry.

WI-38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein a/FAP APC-conjugated Monoclonal Antibody (Catalog # FAB3715A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Fibroblast Activation Protein  alpha /FAP in U87MG cells by Flow Cytometry.

U87MG cells were stained with Mouse Anti-Human Fibroblast Activation Protein  alpha /FAP APC‑conjugated Monoclonal Antibody (Catalog # FAB3715A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Fibroblast Activation Protein alpha/FAP in BxPC3 pancreatic cancer cells by Flow Cytometry.

Detection of Fibroblast Activation Protein alpha/FAP in BxPC3 pancreatic cancer cells by Flow Cytometry.

Used 3uL for 15mins at 4°C on BxPC3 pancreatic cancer cells transduced to over express human FAP. Image from a verified customer review.

Applications

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: WI‑38 human lung fibroblast cell line and U87MG cells

Reviewed Applications

Read 2 reviews rated 5 using FAB3715A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin  alpha 3 beta 1, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.

References

  1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
  2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
  3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
  4. O'Brien, P. and B.F. O'Connor (2008) Biochim. Biophys. Acta 1784:1130.
  5. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

Alternate Names

FAP, Seprase

Entrez Gene IDs

2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)

Gene Symbol

FAP

UniProt

Q12884

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices

For research use only

Citations for Human Fibroblast Activation Protein  alpha /FAP APC‑conjugated Antibody

Customer Reviews (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Fibroblast Activation Protein  alpha /FAP APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human Fibroblast Activation Protein  alpha /FAP APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: BxPC3
    Species: Human
    Verified Customer | Posted 11/17/2025
    Used 3uL for 15mins at 4°C on BxPC3 pancreatic cancer cells transduced to over express human FAP. Other FAP antibodies tested give poor shift but this AF647-conjugated antibody gave an excellent shift allowing us to purify the FAP+ population easily via bead sorting
    Human Fibroblast Activation Protein  alpha /FAP APC‑conjugated Antibody FAB3715A
  • Human Fibroblast Activation Protein  alpha /FAP APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Hs894 CAF cell line, HEK293 human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, U87MG glioblastoma cell line, HEK-FAP transduced cell line, HT1080-FAP transduced cell line, HT-29 human colon adenocarcinoma cell line and KB cervical cancer cell line
    Species: Human
    Verified Customer | Posted 09/24/2023
    Other experimental details provided in: Lindeman, Spencer D., et al. Fibroblast Activation Protein–Targeted Radioligand Therapy for Treatment of Solid Tumors. Journal of Nuclear Medicine 64.5 (2023): 759-766. https://doi.org/10.2967/jnumed.122.264494
    Human Fibroblast Activation Protein  alpha /FAP APC‑conjugated Antibody FAB3715A

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies