Human Fibroblast Activation Protein  alpha /FAP Antibody

Catalog # Availability Size / Price Qty
MAB3715-SP
MAB3715-500
MAB3715-100
Detection of Fibroblast Activation Protein  alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry.
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Product Details
Citations (19)
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Reviews (2)

Human Fibroblast Activation Protein  alpha /FAP Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPP6 and DPP4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 427819
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
Leu26-Asp760
Accession # Q12884
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
WI‑38 human lung fibroblast cell line  and U‑87 MG human glioblastoma/astrocytoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human Fibroblast Activation Protein alpha /FAP Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Fibroblast Activation Protein  alpha /FAP Biotinylated Antibody (Catalog # BAF3715)

Standard: Recombinant Human FAP Protein, CF (Catalog # 3715-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Fibroblast Activation Protein a/FAP antibody in WI-38 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Fibroblast Activation Protein alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry. WI-38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein a/FAP Monoclonal Antibody (Catalog # MAB3715, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of Fibroblast Activation Protein  alpha /FAP in U-87 MG cells by Flow Cytometry. U-87 MG cells were stained with Mouse Anti-Human Fibroblast Activation Protein  alpha /FAP Monoclonal Antibody (Catalog # MAB3715, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin  alpha 3 beta 1, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.

References
  1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
  2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
  3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
  4. O'Brien, P. and B.F. O'Connor (2008) Biochim. Biophys. Acta 1784:1130.
  5. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
Entrez Gene IDs
2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)
Alternate Names
170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin

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Citations for Human Fibroblast Activation Protein  alpha /FAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. The dual role of asporin in breast cancer progression
    Authors: Dana Simkova, Gvantsa Kharaishvili, Gabriela Korinkova, Tomas Ozdian, Tereza Suchánková-Kleplová, Tomas Soukup et al.
    Oncotarget
  2. Fibroblasts Influence Survival and Therapeutic Response in a 3D Co-Culture Model
    Authors: Meher Majety, Leon P. Pradel, Manuela Gies, Carola H. Ries
    PLOS ONE
  3. Cellular census of human fibrosis defines functionally distinct stromal cell types and states
    Authors: TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal
    Nat Commun, 2020-06-02;11(1):2768.
  4. Evaluation of the circulating level of fibroblast activation protein alpha for diagnosis of esophageal squamous cell carcinoma
    Authors: Yuehua Liao, Shan Xing, Banglao Xu, Wanli Liu, Ge Zhang
    Oncotarget
  5. Conventional Cancer Therapies Can Accelerate Malignant Potential of Cancer Cells by Activating Cancer-Associated Fibroblasts in Esophageal Cancer Models
    Authors: Komoto, S;Noma, K;Kato, T;Kobayashi, T;Nishiwaki, N;Narusaka, T;Sato, H;Katsura, Y;Kashima, H;Kikuchi, S;Ohara, T;Tazawa, H;Fujiwara, T;
    Cancers
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Flow Cytometry
  6. Clinical molecular subtyping reveals intrinsic mesenchymal reprogramming in gastric cancer cells
    Authors: Jang, E;Shin, MK;Kim, H;Lim, JY;Lee, JE;Park, J;Kim, J;Kim, H;Shin, Y;Son, HY;Choi, YY;Hyung, WJ;Noh, SH;Suh, JS;Sung, JY;Huh, YM;Cheong, JH;
    Experimental & molecular medicine
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironment
    Authors: H Sato, K Noma, T Ohara, K Kawasaki, M Akai, T Kobayashi, N Nishiwaki, T Narusaka, S Komoto, H Kashima, Y Katsura, T Kato, S Kikuchi, H Tazawa, S Kagawa, Y Shirakawa, H Kobayashi, T Fujiwara
    Oncogene, 2022-11-23;12(1):20152.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Fibroblast activation protein targeted near infrared photoimmunotherapy (NIR PIT) overcomes therapeutic resistance in human esophageal cancer
    Authors: R Katsube, K Noma, T Ohara, N Nishiwaki, T Kobayashi, S Komoto, H Sato, H Kashima, T Kato, S Kikuchi, H Tazawa, S Kagawa, Y Shirakawa, H Kobayashi, T Fujiwara
    Scientific Reports, 2021-01-18;11(1):1693.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  9. A subset of activated fibroblasts is associated with distant relapse in early luminal breast cancer
    Authors: C Bonneau, A Eliès, Y Kieffer, B Bourachot, S Ladoire, F Pelon, D Hequet, JM Guinebreti, C Blanchet, A Vincent-Sa, R Rouzier, F Mechta-Gri
    Breast Cancer Res., 2020-07-14;22(1):76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer
    Authors: Y Kieffer, HR Hocine, G Gentric, F Pelon, C Bernard, B Bourachot, S Lameiras, L Albergante, C Bonneau, A Guyard, K Tarte, A Zinovyev, S Baulande, G Zalcman, A Vincent-Sa, F Mechta-Gri
    Cancer Discov, 2020-05-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Cancer-associated fibroblasts promote immunosuppression by inducing ROS-generating monocytic MDSCs in lung squamous cell carcinoma
    Authors: H Xiang, CP Ramil, J Hai, C Zhang, H Wang, AA Watkins, R Afshar, P Georgiev, MA Sze, XS Song, PJ Curran, M Cheng, JR Miller, D Sun, A Loboda, Y Jia, LY Moy, A Chi, PE Brandish
    Cancer Immunol Res, 2020-02-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Cancer-associated fibroblast heterogeneity in axillary lymph nodes drives metastases in breast cancer through complementary mechanisms
    Authors: F Pelon, B Bourachot, Y Kieffer, I Magagna, F Mermet-Mei, I Bonnet, A Costa, AM Givel, Y Attieh, J Barbazan, C Bonneau, L Fuhrmann, S Descroix, D Vignjevic, P Silberzan, MC Parrini, A Vincent-Sa, F Mechta-Gri
    Nat Commun, 2020-01-21;11(1):404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay
  13. Dissecting Effects of Anti-cancer Drugs and Cancer-Associated Fibroblasts by On-Chip Reconstitution of Immunocompetent Tumor Microenvironments
    Authors: M Nguyen, A De Ninno, A Mencattini, F Mermet-Mei, G Fornabaio, SS Evans, M Cossutta, Y Khira, W Han, P Sirven, F Pelon, D Di Giusepp, FR Bertani, A Gerardino, A Yamada, S Descroix, V Soumelis, F Mechta-Gri, G Zalcman, J Camonis, E Martinelli, L Businaro, MC Parrini
    Cell Rep, 2018-12-26;25(13):3884-3893.e3.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer
    Authors: A Costa, Y Kieffer, A Scholer-Da, F Pelon, B Bourachot, M Cardon, P Sirven, I Magagna, L Fuhrmann, C Bernard, C Bonneau, M Kondratova, I Kuperstein, A Zinovyev, AM Givel, MC Parrini, V Soumelis, A Vincent-Sa, F Mechta-Gri
    Cancer Cell, 2018-02-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture, FACS
  15. Oncolytic adenovirus expressing bispecific antibody targets T-cell cytotoxicity in cancer biopsies
    Authors: JD Freedman, J Hagel, EM Scott, I Psallidas, A Gupta, L Spiers, P Miller, N Kanellakis, R Ashfield, KD Fisher, MR Duffy, LW Seymour
    EMBO Mol Med, 2017-08-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule
    Authors: MA Witek, RD Aufforth, H Wang, JW Kamande, JM Jackson, SR Pullagurla, ML Hupert, J Usary, WZ Wysham, D Hilliard, S Montgomery, V Bae-Jump, LA Carey, PA Gehrig, MI Milowsky, CM Perou, JT Soper, YE Whang, JJ Yeh, G Martin, SA Soper
    NPJ Precis Oncol, 2017-07-25;1(0):.
    Species: Human, Xenograft
    Sample Types: Whole Cells, Whole Tissue
    Applications: Cell Capture, Flow Cytometry, ICC, IHC-P
  17. Bi- and tri-valent T cell engagers deplete tumour-associated macrophages in cancer patient samples
    Authors: Eleanor M. Scott, Egon J. Jacobus, Brian Lyons, Sally Frost, Joshua D. Freedman, Arthur Dyer et al.
    Journal for ImmunoTherapy of Cancer
  18. CD73-Mediated Immunosuppression Is Linked to a Specific Fibroblast Population That Paves the Way for New Therapy in Breast Cancer
    Authors: Ilaria Magagna, Nicolas Gourdin, Yann Kieffer, Monika Licaj, Rana Mhaidly, Pascale Andre et al.
    Cancers (Basel)
  19. Evaluating near‐infrared photoimmunotherapy for targeting fibroblast activation protein‐ alpha expressing cells in vitro and in vivo
    Authors: Jiefu Jin, James D. Barnett, Balaji Krishnamachary, Yelena Mironchik, Catherine K. Luo, Hisataka Kobayashi et al.
    Cancer Science

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Human Fibroblast Activation Protein  alpha /FAP Antibody
By Anonymous on 03/11/2020
Application: ELISA Sample Tested: Recombinant protein Species: Human

Human Fibroblast Activation Protein  alpha /FAP Antibody
By Anonymous on 03/11/2020
Application: ELISA Sample Tested: Human recombinant test antibody Species: Sheep