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Human Fibroblast Activation Protein alpha/FAP Antibody (427819) [PE]

R&D Systems | Catalog # FAB3715P

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry, MACS

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 427819
View all Fibroblast Activation Protein alpha/FAP Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
Leu26-Asp760
Accession # Q12884

Specificity

Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPP6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fibroblast Activation Protein alpha/FAP Antibody (427819) [PE]

Detection of Fibroblast Activation Protein a/FAP antibody in WI‑38 Human Cell Line antibody by Flow Cytometry.

Detection of Fibroblast Activation Protein alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry.

WI-38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein a/FAP PE-conjugated Monoclonal Antibody (Catalog # FAB3715P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Fibroblast Activation Protein  alpha /FAP in U87MG cells by Flow Cytometry.

U87MG cells were stained with Mouse Anti-Human Fibroblast Activation Protein  alpha /FAP PE‑conjugated Monoclonal Antibody (Catalog # FAB3715P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Fibroblast Activation Protein alpha/FAP Antibody (427819) [PE]

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: WI‑38 human lung fibroblast cells and U87MG cells 

Reviewed Applications

Read 1 review rated 5 using FAB3715P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin  alpha 3 beta 1, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.

References

  1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
  2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
  3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
  4. O'Brien, P. and B.F. O'Connor (2008) Biochim. Biophys. Acta 1784:1130.
  5. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

Alternate Names

FAP, Seprase

Entrez Gene IDs

2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)

Gene Symbol

FAP

UniProt

Q12884

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents for Human Fibroblast Activation Protein alpha/FAP Antibody (427819) [PE]

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Product Specific Notices for Human Fibroblast Activation Protein alpha/FAP Antibody (427819) [PE]

For research use only

Citations for Human Fibroblast Activation Protein alpha/FAP Antibody (427819) [PE]

Customer Reviews for Human Fibroblast Activation Protein alpha/FAP Antibody (427819) [PE] (1)

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  • Expression of FAP in transfected CHO-K1 cells
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: CHO Chinese hamster ovary cell line
    Species: Hamster
    Verified Customer | Posted 06/20/2026
    CHOK1 cells transfected with FAP and the cells were incubated with 5 ul of FAP PE (blue) or isotype PE control (red) for 30 min on ice
    CHOK1 cells transfected with FAP and the cells were incubated with 5 ul (1:20) of FAP PE or isotype PE control for 30 min on ice
    Human Fibroblast Activation Protein alpha/FAP Antibody (427819) [PE] FAB3715P
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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