Human Fibroblast Activation Protein  alpha /FAP PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3715P-025
FAB3715P-100
Detection of Fibroblast Activation Protein  alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry.
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Citations (4)
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Human Fibroblast Activation Protein  alpha /FAP PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPP6 is observed.
Source
Monoclonal Mouse IgG1 Clone # 427819
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
Leu26-Asp760
Accession # Q12884
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

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Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Fibroblast Activation Protein  alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry. View Larger

Detection of Fibroblast Activation Protein alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry. WI-38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein a/FAP PE-conjugated Monoclonal Antibody (Catalog # FAB3715P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin  alpha 3 beta 1, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.

References
  1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
  2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
  3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
  4. O'Brien, P. and B.F. O'Connor (2008) Biochim. Biophys. Acta 1784:1130.
  5. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
Entrez Gene IDs
2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)
Alternate Names
170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin

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Citations for Human Fibroblast Activation Protein  alpha /FAP PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. An ex vivo tumor fragment platform to dissect response to PD-1 blockade in cancer
    Authors: P Voabil, M de Bruijn, LM Roelofsen, SH Hendriks, S Brokamp, M van den Br, A Broeks, J Sanders, P Herzig, A Zippelius, CU Blank, KJ Hartemink, K Monkhorst, JBAG Haanen, TN Schumacher, DS Thommen
    Nature Medicine, 2021;27(7):1250-1261.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony
    Authors: D Ali, D Alhattab, H Jafar, M Alzubide, N Sharar, S Bdour, A Awidi
    International Journal of Molecular Sciences, 2021;22(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Destabilization of NOXA mRNA as a common resistance mechanism to targeted therapies
    Authors: J Montero, C Gstalder, DJ Kim, D Sadowicz, W Miles, M Manos, JR Cidado, J Paul Secri, AE Tron, K Flaherty, F Stephen Ho, CH Yoon, A Letai, DE Fisher, R Haq
    Nat Commun, 2019;10(1):5157.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Isolation of Fibroblast-Activation Protein-Specific Cancer-Associated Fibroblasts
    Authors: Y Huang, S Zhou, Y Huang, D Zheng, Q Mao, J He, Y Wang, D Xue, X Lu, N Yang, Y Zhao
    Biomed Res Int, 2017;2017(0):4825108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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