Human Fibroblast Activation Protein  alpha /FAP PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPP6 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 427819
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
    Accession # Q12884
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Fibroblast Activation Protein  alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry. WI‑38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein  alpha /FAP PE‑conjugated Monoclonal Antibody (Catalog # FAB3715P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin  alpha 3 beta 1, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.

  • References:
    1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
    2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
    3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
    4. O'Brien, P. and B.F. O'Connor (2008) Biochim. Biophys. Acta 1784:1130.
    5. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
    6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
  • Entrez Gene IDs:
    2191 (Human); 14089 (Mouse)
  • Alternate Names:
    170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast activation protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; seprase
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Isotype Controls
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Mouse IgG1 PE-conjugated Antibody

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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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