natural and recombinant human Fibronectin and Fibronectin fragments aa 1-1908, aa 607-1265, aa 627-702. Fibronectin fragment aa 631-705 cross-reacts approximately 0.415% Fibronectin fragments aa 1266-1908, aa 1722-1811, and aa 1913-2477 are not detected in this kit.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Human Fibronectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fibronectin in serum-free cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
The recovery of human Fibronectin spiked to levels throughout the range of the assay was evaluated.
Average % Recovery
Cell Culture Media (n=4)
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Fibronectin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions, and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, fibronectin is highly adhesive when immobilized on a surface.