Human FOLR1 Alexa Fluor® 647-conjugated Antibody

    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 548908
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
    Arg25-Met233
    Accession # P15328
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of FOLR1 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB5646R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FOLR1
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).
  • References:
    1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
    2. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
    3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
    4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
    5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
    6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
    7. Ross, J.F. et al. (1994) Cancer 73:2432.
    8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
    9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
    10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
    11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.
  • Long Name:
    Folate Receptor 1
  • Entrez Gene IDs:
    2348 (Human); 14275 (Mouse); 171049 (Rat)
  • Alternate Names:
    Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

Ctrl IC002R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human FOLR1 Alexa Fluor® 647-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee