Human FOLR1 Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 548908
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
    Arg25-Met233
    Accession # P15328
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
    • Human FOLR1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human FOLR1 Antibody (Catalog # MAB5646)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human FOLR1 Biotinylated Antibody (Catalog # BAF5646)
  • ELISA Standard
     
    Recombinant Human FOLR1 Protein, CF (Catalog # 5646-FR)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of FOLR1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti‑Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Detection of Human FOLR1 by Western Blot. Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FOLR1 at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. Use under non-reducing conditions only.
Immunocytochemistry
FOLR1 in MCF-7 Human Cell Line. FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FOLR1
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).
  • References:
    1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
    2. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
    3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
    4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
    5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
    6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
    7. Ross, J.F. et al. (1994) Cancer 73:2432.
    8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
    9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
    10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
    11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.
  • Long Name:
    Folate Receptor 1
  • Entrez Gene IDs:
    2348 (Human); 14275 (Mouse); 171049 (Rat)
  • Alternate Names:
    Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Promising Nanocarriers for PEDF Gene Targeting Delivery to Cervical Cancer Cells Mediated by the Over-expressing FR?
    Sci Rep, 2016;6(0):32427.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Conditions associated with circulating tumor-associated folate receptor 1 protein in healthy men and women.
    Authors: Kelemen L, Brenton J, Parkinson C, C Whitaker H, Piskorz A, Csizmadi I, Robson P
    PLoS ONE, 2014;9(5):e96542.
    Species: Human
    Sample Type: Serum
    Application: ELISA Capture
  3. Harnessing engineered antibodies of the IgE class to combat malignancy: initial assessment of FcvarepsilonRI-mediated basophil activation by a tumour-specific IgE antibody to evaluate the risk of type I hypersensitivity.
    Authors: Rudman SM, Josephs DH, Cambrook H, Karagiannis P, Gilbert AE, Dodev T, Hunt J, Koers A, Montes A, Taams L, Canevari S, Figini M, Blower PJ, Beavil AJ, Nicodemus CF, Corrigan C, Kaye SB, Nestle FO, Gould HJ, Spicer JF, Karagiannis SN
    Clin. Exp. Allergy, 2011;41(10):1400-1413.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Average Rating: 1 (Based on 1 review)

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We have 1 review tested in 1 application: Western Blot.

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Western Blot Human FOLR1 Antibody MAB5646
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 WB Human Anonymous 02/23/2016
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Western Blot Human FOLR1 Antibody MAB5646
Western Blot: Human FOLR1 Antibody [MAB5646]

Summary

ApplicationWestern Blot
Sample TestedOvarian cancer tissue
SpeciesHuman

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