Human FOLR1 Antibody

Catalog # Availability Size / Price Qty
MAB5646
MAB5646-SP
Detection of Human FOLR1 by Western Blot.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (6)

Human FOLR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 548908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
Arg25-Met233
Accession # P15328
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
FOLR1 is specifically detected in MCF-7 human breast cancer parental cell line but is not detectable in FOLR1 knockout MCF-7 cell line.
 

Human FOLR1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human FOLR1 Biotinylated Antibody (Catalog # BAF5646)

Standard: Recombinant Human FOLR1 Protein, CF (Catalog # 5646-FR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human FOLR1 antibody by Western Blot. View Larger

Detection of Human FOLR1 by Western Blot. Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FOLR1 at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. Use under non-reducing conditions only.

Flow Cytometry Detection of FOLR1 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of FOLR1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry FOLR1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

FOLR1 in MCF-7 Human Cell Line. FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated FOLR1  Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

FOLR1 Specificity is Shown by Flow Cytometry in Knockout Cell Line. FOLR1 knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the FOLR1 knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).

References
  1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  2. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
  4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
  5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
  6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
  9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
  11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.
Long Name
Folate Receptor 1
Entrez Gene IDs
2348 (Human); 14275 (Mouse); 171049 (Rat)
Alternate Names
Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18

Product Datasheets

Citations for Human FOLR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays
    Authors: J Song, LJ Sokoll, DW Chan, Z Zhang
    Biomedicines, 2021;9(12):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  2. Efficient delivery of small interfering RNAs targeting particular mRNAs into pancreatic cancer cells inhibits invasiveness and metastasis of pancreatic tumors
    Authors: K Taniuchi, T Yawata, M Tsuboi, T Ueba, T Saibara
    Oncotarget, 2019;10(30):2869-2886.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Promising Nanocarriers for PEDF Gene Targeting Delivery to Cervical Cancer Cells Mediated by the Over-expressing FR?
    Sci Rep, 2016;6(0):32427.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Conditions associated with circulating tumor-associated folate receptor 1 protein in healthy men and women.
    Authors: Kelemen L, Brenton J, Parkinson C, C Whitaker H, Piskorz A, Csizmadi I, Robson P
    PLoS ONE, 2014;9(5):e96542.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  5. Harnessing engineered antibodies of the IgE class to combat malignancy: initial assessment of FcvarepsilonRI-mediated basophil activation by a tumour-specific IgE antibody to evaluate the risk of type I hypersensitivity.
    Authors: Rudman SM, Josephs DH, Cambrook H, Karagiannis P, Gilbert AE, Dodev T, Hunt J, Koers A, Montes A, Taams L, Canevari S, Figini M, Blower PJ, Beavil AJ, Nicodemus CF, Corrigan C, Kaye SB, Nestle FO, Gould HJ, Spicer JF, Karagiannis SN
    Clin. Exp. Allergy, 2011;41(10):1400-1413.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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Secondary Antibodies

Reviews for Human FOLR1 Antibody

Average Rating: 3.5 (Based on 6 Reviews)

5 Star
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4 Star
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2 Star
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Human FOLR1 Antibody
By Anonymous on 08/26/2021
Application: WB Sample Tested: Human cancer cell line Species: Human

Human FOLR1 Antibody
By Anonymous on 04/10/2019
Application: Flow Sample Tested: IGROV1 cell line Species: Human

Human FOLR1 Antibody
By Anonymous on 10/04/2018
Application: WB Sample Tested: Human cancer cell line Species: Human

After very long exposure gave a band of an expected size. Very weak signal even with ECL Plus.


Human FOLR1 Antibody
By Anonymous on 05/08/2018
Application: WB Sample Tested: CAOV3,HT-29 human colon adenocarcinoma cell line,igrov-1,NCI-H292,SK-OV-3 human ovarian adenocarcinoma cell line Species: Human

Human FOLR1 Antibody
By Anonymous on 04/03/2018
Application: Flow Sample Tested: human cancer cell line Species: Human

Human FOLR1 Antibody
By Anonymous on 02/23/2016
Application: WB Sample Tested: Ovarian cancer tissue Species: Human

R&D Systems Technical Service will investigate.