Human FOLR1 PE-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 548908
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
    Arg25-Met233
    Accession # P15328
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of FOLR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FOLR1
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the blood and breast milk (11).
  • References:
    1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
    2. Fowler, B. (2001) Kidney Int. Suppl. 78:S221.
    3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
    4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
    5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
    6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
    7. Ross, J.F. et al. (1994) Cancer 73:2432.
    8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
    9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
    10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
    11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.
  • Long Name:
    Folate Receptor 1
  • Entrez Gene IDs:
    2348 (Human); 14275 (Mouse); 171049 (Rat)
  • Alternate Names:
    Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Potent Therapeutic Activity Against Peritoneal Dissemination and Malignant Ascites by the Novel Anti-Folate Receptor Alpha Antibody KHK2805
    Authors: M Ando, K Nagata, K Nihira, Y Suzuki, Y Kanda, M Adachi, T Kubota, N Kameyama, M Nakano, H Ando, K Yamano, T Ishii, R Nakai, K Nakamura
    Transl Oncol, 2017;10(5):707-718.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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