Human FOLR1 PE-conjugated Antibody
Human FOLR1 PE-conjugated Antibody Summary
Arg25-Met233
Accession # P15328
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of FOLR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of FOLR1 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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FOLR1 Specificity is Shown by Flow Cytometry in Knockout Cell Line. FOLR1 knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the FOLR1 knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FOLR1
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the blood and breast milk (11).
- Kelemen, L.E. (2006) Int. J. Cancer 119:243.
- Fowler, B. (2001) Kidney Int. Suppl. 78:S221.
- Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
- Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
- Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
- Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
- Ross, J.F. et al. (1994) Cancer 73:2432.
- Parker, N. et al. (2005) Anal. Biochem. 338:284.
- Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
- Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
- Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.
Product Datasheets
Citations for Human FOLR1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Nanotechnology-Based Cisplatin Intracellular Delivery to Enhance Chemo-Sensitivity of Ovarian Cancer
Authors: B Bortot, M Mongiat, E Valencic, S Dal Monego, D Licastro, M Crosera, G Adami, E Rampazzo, G Ricci, F Romano, GM Severini, S Biffi
Int J Nanomedicine, 2020;15(0):4793-4810.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Identification of differentially expressed genes in lung adenocarcinoma cells using single-cell RNA sequencing not detected using traditional RNA sequencing and microarray
Authors: Z Chen, M Zhao, M Li, Q Sui, Y Bian, J Liang, Z Hu, Y Zheng, T Lu, Y Huang, C Zhan, W Jiang, Q Wang, L Tan
Lab. Invest., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Nanotechnology-Based Cisplatin Intracellular Delivery to Enhance Chemo-Sensitivity of Ovarian Cancer
Authors: B Bortot, M Mongiat, E Valencic, S Dal Monego, D Licastro, M Crosera, G Adami, E Rampazzo, G Ricci, F Romano, GM Severini, S Biffi
Int J Nanomedicine, 2020;15(0):4793-4810.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Potent Therapeutic Activity Against Peritoneal Dissemination and Malignant Ascites by the Novel Anti-Folate Receptor Alpha Antibody KHK2805
Authors: M Ando, K Nagata, K Nihira, Y Suzuki, Y Kanda, M Adachi, T Kubota, N Kameyama, M Nakano, H Ando, K Yamano, T Ishii, R Nakai, K Nakamura
Transl Oncol, 2017;10(5):707-718.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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