Human FOLR1 Quantikine ELISA Kit

  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (10 uL), Urine (10 uL), Human Milk (10 uL)
  • Sensitivity
    14.9 pg/mL
  • Assay Range
    46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
  • Specificity
    Natural and recombinant human FOLR1. This assay recognizes recombinant human FOLR1 in the presence of folic acid.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC92, Quantikine Immunoassay Control Set 724 for Human FOLR1 - Please Inquire
Product Summary
The Quantikine Human FOLR1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FOLR1 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human FOLR1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FOLR1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FOLR1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean3771024192239010501973
Standard Deviation9.2819.829.520.364135
CV%2.51.91.55.26.16.9

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean3771024192239010501973
Standard Deviation9.2819.829.520.364135
CV%2.51.91.55.26.16.9

Recovery

The recovery of FOLR1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 94-107
EDTA Plasma (n=4) 95 84-106
Heparin Plasma (n=4) 96 82-105
Serum (n=4) 96 84-107
Linearity
To assess the linearity of the assay, samples containing high concentrations of FOLR1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FOLR1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37-42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. Human FOLR1 shares 83 percent amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up-regulated on many carcinomas. It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk.

    • Long Name
      Folate Receptor 1
    • Entrez Gene IDs
      2348 (Human); 14275 (Mouse); 171049 (Rat);
    • Alternate Names
      Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 724 for Human FOLR1

    Ctrl QC92
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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