Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit

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Human Galectin-3BP/MAC-2BP ELISA Standard Curve
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Product Details
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Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)
0.161 ng/mL
Assay Range
0.4 - 25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Natural and recombinant human Galectin-3BP/MAC-2BP
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Galectin-3BP/MAC-2BP Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3BP in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human Galectin-3BP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human Galectin-3BP. Results obtained using natural human Galectin-3BP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3BP.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 32 32 32
Mean (pg/mL) 0.743 2.94 8.27 0.778 3.2 9.1
Standard Deviation 0.027 0.08 0.244 0.078 0.225 0.434
CV% 3.6 2.7 3 10 7 4.8


The recovery of human Galectin-3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 99 95-101
Saliva (n=4) 91 82-101
Urine (n=4) 104 98-111


To assess the linearity of the assay, samples containing high concentrations of Galectin-3BP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Human Galectin-3BP/MAC-2BP ELISA Linearity

Scientific Data

Human Galectin-3BP/MAC-2BP ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-3BP/MAC-2BP

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Long Name:
Galectin-3 Binding Protein
Entrez Gene IDs:
3959 (Human)
Alternate Names:
90K; Basement membrane autoantigen p105; BTBD17B; galectin-3-binding protein; Galectin3BP; Galectin-3BP; L3 antigen; Lectin galactoside-binding soluble 3-binding protein; lectin, galactoside-binding, soluble, 3 binding protein; LGALS3BP; M2BP; Mac-2 BP; Mac-2-binding protein; MAC2BP; MAC-2BP; MAC-2-BP; serum protein 90K; Tumor-associated antigen 90K
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Glycosphingolipid Levels in Urine Extracellular Vesicles Enhance Prediction of Therapeutic Response in Lupus Nephritis
    Authors: B Troyer, J Rodgers, BJ Wolf, JC Oates, RR Drake, TK Nowling
    Metabolites, 2022-02-01;12(2):.
    Species: Human
    Sample Types: Urine
  2. SARS-CoV-2 RNAemia and proteomic trajectories inform prognostication in COVID-19 patients admitted to intensive care
    Authors: C Gutmann, K Takov, SA Burnap, B Singh, H Ali, K Theofilato, E Reed, M Hasman, A Nabeebaccu, M Fish, MJ McPhail, K O'Gallaghe, LE Schmidt, C Cassel, M Rienks, X Yin, G Auzinger, S Napoli, SF Mujib, F Trovato, B Sanderson, B Merrick, U Niazi, M Saqi, K Dimitrakop, R Fernández-, S Braun, R Kronstein-, KJ Doores, JD Edgeworth, AM Shah, SR Bornstein, T Tonn, AC Hayday, M Giacca, M Shankar-Ha, M Mayr
    Nature Communications, 2021-06-07;12(1):3406.
    Species: Human
    Sample Types: Plasma
  3. Investigating LGALS3BP/90?K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
    Authors: J Costa, A Pronto-Lab, S Pinto, M Gromicho, S Bonucci, E Tranfield, C Correia, BM Alexandre, M de Carvalh
    Sci Rep, 2020-03-27;10(1):5649.
    Species: Human
    Sample Types: CSF
  4. Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
    Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
    J. Clin. Invest, 2017-03-20;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
    Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
    Sci Rep, 2017-01-23;7(0):40994.
    Species: Human
    Sample Types: Serum


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