Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit

R&D Systems | Catalog # DGBP30B

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL)

Sensitivity

0.161 ng/mL

Assay Range

0.391-25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
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Product Summary for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit

The Quantikine Human Galectin-3BP/MAC-2BP Immunoassay is a 4.5 hour solid phase ELISA designed to measure Galectin-3BP in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human Galectin-3BP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human Galectin-3BP. Results obtained using natural human Galectin-3BP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Galectin-3BP.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Galectin-3BP/MAC-2BP

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 32 32 32
Mean (pg/mL) 0.743 2.94 8.27 0.778 3.2 9.10
Standard Deviation 0.027 0.080 0.244 0.078 0.225 0.434
CV% 3.6 2.7 3.0 10.0 7.0 4.8

Recovery for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit

The recovery of human Galectin-3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 99 95-101
Saliva (n=4) 91 82-101
Urine (n=4) 104 98-111

Linearity

To assess the linearity of the assay, samples containing high concentrations of Galectin-3BP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Human Galectin-3BP/MAC-2BP ELISA Linearity

Scientific Data Images for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit

Human Galectin-3BP/MAC-2BP ELISA Standard Curve

Human Galectin-3BP/MAC-2BP ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-3BP/MAC-2BP

Galectin-3 binding protein, also known as MAC-2BP, M2BP, and 90K, is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily. Galectin-3BP binds Galectin-3 with high affinity, and additionally binds Galectins -1 and -7, several Collagen types, Fibronectin, beta 1 Integrins, and Nidogen. It is widely expressed in extracellular fluids and in pericellular areas of cell-rich tissues. Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers. It has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity. Galectin-3BP upregulation has also been correlated with tumor aggressiveness.

 

Long Name

Galectin-3 Binding Protein

Alternate Names

90K, Galectin3BP, LGALS3BP, MAC-2BP

Entrez Gene IDs

3959 (Human)

Gene Symbol

LGALS3BP

Additional Galectin-3BP/MAC-2BP Products

Product Documents for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit

Customer Reviews for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit (3)

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  • Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 09/13/2020
  • Name: Anonymous
    Sample Tested: HASMC human aortic smooth muscle cells
    Verified Customer | Posted 10/08/2018
  • Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
    Name: Christian Cassel
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 08/29/2018

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