Human Galectin-9 Quantikine ELISA Kit

R&D Systems | Catalog # DGAL90

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (100 µL), Urine (10 µL), Human Milk (10 µL)

Sensitivity

0.028 ng/mL

Assay Range

0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
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Product Summary for Human Galectin-9 Quantikine ELISA Kit

The Quantikine Human Galectin-9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-9 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Galectin-9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Galectin-9.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Galectin-9.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Human Milk, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.34 3.58 6.13 1.44 3.43 6.05
Standard Deviation 0.05 0.12 0.22 0.08 0.18 0.32
CV% 3.7 3.4 3.6 5.6 5.2 5.3

EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.44 3.77 6.29 1.40 3.28 5.84
Standard Deviation 0.06 0.07 0.08 0.11 0.20 0.30
CV% 4.2 1.9 1.3 7.9 6.1 5.1

Recovery for Human Galectin-9 Quantikine ELISA Kit

The recovery of human Galectin-9 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 84-104
EDTA Plasma (n=4) 103 85-117
Heparin Plasma (n=4) 104 89-117
Serum (n=4) 106 86-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Galectin-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Galectin-9 ELISA Linearity

Scientific Data Images for Human Galectin-9 Quantikine ELISA Kit

Human Galectin-9 ELISA Serum/Plasma/Urine Standard Curve

Human Galectin-9 ELISA Serum/Plasma/Urine Standard Curve

Human Galectin-9 ELISA Cell Culture Supernate/Saliva/Milk Standard Curve

Human Galectin-9 ELISA Cell Culture Supernate/Saliva/Milk Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-9

Galectin-9 is a secreted carbohydrate-binding protein with two lectin domains connected by a linker region. Progressive deletion within the linker region generates an isoform known as Ecalectin or UAT. Galectin-9 binds to carbohydrate moieties of IgE, preventing immune complex formation, mast cell degranulation, and asthmatic and cutaneous anaphylaxis reactions. It functions as an eosinophil chemoattractant and induces the maturation of dendritic cells which promote Th1 polarization. Galectin-9 binds to TIM-3, inhibiting Th1 cell and CD8+ T cell responses but promoting Treg differentiation and activity. Galectin-9 suppresses tumor cell metastasis by interfering with the associations between hyaluronic acid and CD44 and between VCAM-1 and Integrin alpha4 beta1. The Ecalectin isoform (UAT; urate transporter) can be expressed as an integral membrane protein and mediate the cellular efflux of urate.

Alternate Names

Ecalectin, GAL9, Galectin9, LGALS9

Entrez Gene IDs

3965 (Human); 16859 (Mouse)

Gene Symbol

LGALS9

Additional Galectin-9 Products

Product Documents for Human Galectin-9 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Galectin-9 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Galectin-9 Quantikine ELISA Kit

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  • Human Galectin-9 Quantikine ELISA Kit
    Name: Adam Guess
    Sample Tested: Mesenchymal stem cells
    Verified Customer | Posted 06/21/2016
    Human Galectin-9 Quantikine ELISA Kit DGAL90

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Protocols

View specific protocols for Human Galectin-9 Quantikine ELISA Kit (DGAL90):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. PROTECT FROM LIGHT.
  9.   For Cell Culture Supernate, Saliva, or Human Milk Samples: Incubate at room temperature for 20 minutes on the benchtop.
    For Serum, Plasma, or Urine Samples: Incubate at room temperature for 30 minutes on the benchtop.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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