Human Galectin-9 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Saliva, Human Milk
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean | 1.34 | 3.58 | 6.13 | 1.44 | 3.43 | 6.05 |
Standard Deviation | 0.05 | 0.12 | 0.22 | 0.08 | 0.18 | 0.32 |
CV% | 3.7 | 3.4 | 3.6 | 5.6 | 5.2 | 5.3 |
Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean | 1.44 | 3.77 | 6.29 | 1.4 | 3.28 | 5.84 |
Standard Deviation | 0.06 | 0.07 | 0.08 | 0.11 | 0.2 | 0.3 |
CV% | 4.2 | 1.9 | 1.3 | 7.9 | 6.1 | 5.1 |
Recovery
The recovery of human Galectin-9 spiked to levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 96 | 84-104 |
EDTA Plasma (n=4) | 103 | 85-117 |
Heparin Plasma (n=4) | 104 | 89-117 |
Serum (n=4) | 106 | 86-117 |
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background: Galectin-9
The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. PROTECT FROM LIGHT.
- For Cell Culture Supernate, Saliva, or Human Milk Samples: Incubate at room temperature for 20 minutes on the benchtop.
For Serum, Plasma, or Urine Samples: Incubate at room temperature for 30 minutes on the benchtop. - Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.





Citations for Human Galectin-9 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Aspergillus�fumigatus enhances human NK cell activity by regulating M1 macrophage polarization
Authors: X Zhang, D He, S Gao, Y Wei, L Wang
Mol Med Rep, 2019;20(2):1241-1249.
Species: Human
Sample Types: Cell Culture Supernates
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Clinical Significance of Serum Galectin-9 and Soluble CD155 Levels in Patients with Systemic Sclerosis
Authors: M Chihara, M Kurita, Y Yoshihara, A Asahina, K Yanaba
J Immunol Res, 2018;2018(0):9473243.
Species: Human
Sample Types: Serum
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Expression profiles and clinical value of plasma exosomal Tim-3 and Galectin-9 in non-small cell lung cancer
Authors: J Gao, X Qiu, X Li, H Fan, F Zhang, T Lv, Y Song
Biochem. Biophys. Res. Commun., 2018;0(0):.
Species: Human
Sample Types: Plasma
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Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
Sci Rep, 2017;7(0):40994.
Species: Human
Sample Types: Serum
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TIM-3 plays a more important role than PD-1 in the functional impairments of cytotoxic T cells of malignant Schwannomas
Authors: Z Li, X Liu, R Guo, P Wang
Tumour Biol., 2017;39(5):1010428317698.
Species: Human
Sample Types: Cell Culture Supernates
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Increased peripheral cd4+ regulatory t cells persist after successful direct-acting antiviral treatment of chronic hepatitis C
Authors: Bettina Langhans
J. Hepatol, 2016;0(0):.
Species: Human
Sample Types: Serum
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Human Galectin-9 Is a Potent Mediator of HIV Transcription and Reactivation
Authors: Mohamed Abdel-Mohs
PLoS Pathog, 2016;12(6):e1005677.
Species: Human
Sample Types: Plasma
FAQs
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