Human Galectin-9 Quantikine ELISA Kit
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Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Galectin-9 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (100 uL), Urine (10 uL), Human Milk (10 uL)
Sensitivity
0.028 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human Galectin-9.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Galectin-9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-9 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E.
coli-expressed recombinant human Galectin-9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Galectin-9.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Saliva, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.34 | 3.58 | 6.13 | 1.44 | 3.43 | 6.05 |
| Standard Deviation | 0.05 | 0.12 | 0.22 | 0.08 | 0.18 | 0.32 |
| CV% | 3.7 | 3.4 | 3.6 | 5.6 | 5.2 | 5.3 |
Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.44 | 3.77 | 6.29 | 1.4 | 3.28 | 5.84 |
| Standard Deviation | 0.06 | 0.07 | 0.08 | 0.11 | 0.2 | 0.3 |
| CV% | 4.2 | 1.9 | 1.3 | 7.9 | 6.1 | 5.1 |
Recovery
The recovery of human Galectin-9 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 84-104 |
| EDTA Plasma (n=4) | 103 | 85-117 |
| Heparin Plasma (n=4) | 104 | 89-117 |
| Serum (n=4) | 106 | 86-117 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Galectin-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Data Examples
Product Datasheets
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Galectin-9
The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.
Entrez Gene IDs:
3965 (Human); 16859 (Mouse)
Alternate Names:
Ecalectin; GAL9; gal-9; galectin 9; Galectin9; Galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. PROTECT FROM LIGHT.
- For Cell Culture Supernate, Saliva, or Human Milk Samples: Incubate at room temperature for 20 minutes on the benchtop.
For Serum, Plasma, or Urine Samples: Incubate at room temperature for 30 minutes on the benchtop. - Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
100 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human Galectin-9 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Clinical Significance of Serum Galectin-9 and Soluble CD155 Levels in Patients with Systemic Sclerosis
Authors: M Chihara, M Kurita, Y Yoshihara, A Asahina, K Yanaba
J Immunol Res, 2018;2018(0):9473243.
Species: Human
Sample Types: Serum
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Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
Sci Rep, 2017;7(0):40994.
Species: Human
Sample Types: Serum
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TIM-3 plays a more important role than PD-1 in the functional impairments of cytotoxic T cells of malignant Schwannomas
Authors: Z Li, X Liu, R Guo, P Wang
Tumour Biol., 2017;39(5):1010428317698.
Species: Human
Sample Types: Cell Culture Supernates
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Increased peripheral cd4+ regulatory t cells persist after successful direct-acting antiviral treatment of chronic hepatitis C
Authors: Bettina Langhans
J. Hepatol, 2016;0(0):.
Species: Human
Sample Types: Serum
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Human Galectin-9 Is a Potent Mediator of HIV Transcription and Reactivation
Authors: Mohamed Abdel-Mohs
PLoS Pathog, 2016;12(6):e1005677.
Species: Human
Sample Types: Plasma
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Human Galectin-9 Quantikine ELISA Kit
By Anonymous on 06/21/2016
Sample Tested: Mesenchymal stem cells
