Human GASP-2/WFIKKN Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM21362
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Human GASP-2/WFIKKN Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GASP‑2/WFIKKN in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human GASP-1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 289839
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GASP-2/WFIKKN
Ala20-Asp548
Accession # Q96NZ8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human GASP-2/WFIKKN Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human GASP‑2/WFIKKN Antibody (Catalog # MAB21361)

Standard: Recombinant Human GASP-2/WFIKKN Protein (Catalog # 2136-GS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GASP-2/WFIKKN

Growth and differentiation factor-associated serum protein-2 (GASP-2) cDNA encodes a 548 amino acid protein that contains a 19 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Based on the order of these conserved domains, GASP-2 is also known as WFIKKN (1). Another related protein which contains the same domain structure is called WFIKKNRP (WFIKKN-related protein), or GASP-1 (2, 3). WAP, follistatin, Kunitz and netrin domains are all implicated in protease inhibition, and the GASP proteins may be multivalent protease inhibitors (1, 4). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-2 is abundant in the lung, skeletal muscle and liver while GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney (3). In the adult, GASP-2 is expressed primarily in the pancreas, liver, and thymus while GASP-1 is in the ovary, testis, and brain (3). Further characterization shows that GASP-1 inhibits myostatin (GDF-8) and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (2). Although, this kind of activity has not been reported for GASP-2, tests at R&D Systems have determined that GASP-2 shows similar inhibitory activity towards myostatin as GASP-1. By amino acid sequence, human GASP-2 is 55% identical to human GASP-1.

References
  1. Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3705.
  2. Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
  3. Trexler, M. et al. (2002) Biol. Chem. 383:223.
  4. Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
Long Name
Growth and Differentiation Factor-associated Serum Protein-2
Entrez Gene IDs
117166 (Human); 215001 (Mouse)
Alternate Names
C16orf12; Chromosome 16 Open Reading Frame 12; GASP2; GASP-2; Growth And Differentiation Factor-Associated Serum Protein 2; hGASP-2; RJD2; WAP Four-Disulfide Core Domain 20A; WAP, Follistatin/Kazal, Immunoglobulin, Kunitz And Netrin Domain Containing 1; WAP, FS, Ig, KU, And NTR-Containing Protein; WFDC20A; WFIKKN; WFIKKN1

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